Structure

    redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p$MONO,Und,-H,0,redEnd

Rainbow trout mucus O-glycosylation

GlyTouCan Accession Number: Loading...

Peak Intensity
233.95 0.2
241.04 0.6
262.12 52.8
262.8 0.5
263.16 3.7
263.73 0.0
293.34 0.3
298.98 0.4
311.3 0.5
316.91 0.1
317.18 1.0
317.75 0.0
341.37 0.1
347.24 1.4
347.81 0.0
352.03 0.3
364.12 0.1

Annotations:

BY ?1D-GalNAc,p/#bcleavage--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
366.06 1.2
366.62 0.0
367.3 0.2
384.24 3.7
384.64 0.2
385.18 0.4
385.42 0.2
387.19 1.0

Annotations:

BZ ?1D-GalNAc,p/#bcleavage--??1HexNAc,p(--??1D-Gal,p/#zcleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
387.64 0.4

Annotations:

BZ ?1D-GalNAc,p/#bcleavage--??1HexNAc,p(--??1D-Gal,p/#zcleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
388.27 0.5

Annotations:

ZZ redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p/#zcleavage)--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
388.95 0.2

Annotations:

ZZ redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p/#zcleavage)--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
389.3 0.2

Annotations:

ZZ redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p/#zcleavage)--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
405.29 11.4

Annotations:

BY ?1D-GalNAc,p/#bcleavage--??1HexNAc,p(--??1D-Gal,p/#ycleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--??1HexNAc,p(--??1D-Gal,p/#zcleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
406.22 0.6

Annotations:

YZ redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p/#ycleavage)--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p/#zcleavage)--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
406.43 0.5

Annotations:

YZ redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p/#ycleavage)--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p/#zcleavage)--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
406.93 0.9

Annotations:

YZ redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p/#ycleavage)--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p/#zcleavage)--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
407.24 1.3

Annotations:

YZ redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p/#ycleavage)--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p/#zcleavage)--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
407.82 0.0

Annotations:

YZ redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p/#ycleavage)--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p/#zcleavage)--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
408.29 0.3
410.1 0.8
410.33 0.3
422.97 0.1

Annotations:

CY ?1D-GalNAc,p/#ccleavage--??1HexNAc,p(--??1D-Gal,p/#ycleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
424.23 0.6

Annotations:

YY redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p/#ycleavage)--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
425.38 1.2

Annotations:

YY redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p/#ycleavage)--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
425.98 0.0

Annotations:

YY redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p/#ycleavage)--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
479.53 0.3
509.32 0.2
521.35 0.7
522.32 0.3
525.33 0.1
527.03 0.3
527.55 0.2
528.13 0.3
528.5 0.1
551.34 0.3
555.2 0.6
567.2 1.1

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p$MONO,Und,-H,0,redEnd
567.47 1.2

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p$MONO,Und,-H,0,redEnd
568.62 1.5

Annotations:

Z redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
569.31 3.7

Annotations:

Z redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
569.95 0.0

Annotations:

Z redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
571.47 0.2
587.52 0.6

Annotations:

Y redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
588.28 2.6
588.84 0.0
589.45 0.6
592.33 0.2
593.87 0.2
610.03 1.1

Annotations:

Z redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p/#zcleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
610.31 0.3

Annotations:

Z redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p/#zcleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
613.47 0.1
618.59 0.3
622.17 0.2
625.49 0.1
628.27 0.7

Annotations:

Y redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p/#ycleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
629.33 0.2
630.22 0.2
630.44 0.3
640.54 0.6
641.17 0.6
670.54 0.3
682.33 0.5
683.31 0.3
683.63 0.1
688.63 0.8
692.43 0.5
699.31 0.3
701.27 0.3
701.58 0.6
709.54 0.1
715.31 0.4
729.63 0.5
730.49 17.8
731.58 4.4
732.17 0.0
733.24 0.4
739.91 0.2
747.56 0.1
748.13 0.4
748.4 0.3
748.7 0.5
749.65 0.3
753.36 0.1

There are 6 similar spectra:

Id Structure Score

7406

0.92

7435

0.92

5431

0.84

5717

0.80

4806

0.76

0.76

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 789.32
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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