Structure

    redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1L-Fuc,p)--??1D-GlcNAc,p--??1D-Gal,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd

The intestinal O-glycome is modified by reactive oxygen species deficiency

GlyTouCan Accession Number: Loading...

Peak Intensity
307.11 4.2

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1D-Gal,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd B ?1D-GlcNAc,p/#bcleavage--??1D-Gal,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
325.0 4.3

Annotations:

C ?1D-GalNAc,p/#ccleavage--??1D-Gal,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd C ?1D-GlcNAc,p/#ccleavage--??1D-Gal,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
347.29 1.7
348.1 2.8

Annotations:

ZZ redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1L-Fuc,p/#zcleavage)--??1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
371.3 5.8
389.2 98.5

Annotations:

ZZ redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??1D-GlcNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
390.18 13.2
392.22 5.0
406.3 2.4
407.23 45.7

Annotations:

YZ redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??1D-GlcNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
408.31 5.1
470.28 4.5
503.43 3.7
509.25 1.4
510.3 30.5

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1D-GlcNAc,p--??1D-Gal,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
511.31 4.1
512.27 39.0

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1L-Fuc,p)--??1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
513.43 4.5
527.42 1.4
530.3 35.3

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1L-Fuc,p)--??1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
531.29 9.5
551.32 41.5

Annotations:

ZZ redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1L-Fuc,p/#zcleavage)--??1D-GlcNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd ZZ redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??1D-GlcNAc,p--??1D-Gal,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
552.07 2.7
552.33 6.8
553.37 1.9
565.44 1.4
569.24 14.1

Annotations:

YZ redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1L-Fuc,p/#ycleavage)--??1D-GlcNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1L-Fuc,p/#zcleavage)--??1D-GlcNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p--??1D-Gal,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??1D-GlcNAc,p--??1D-Gal,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
569.65 2.5

Annotations:

YZ redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1L-Fuc,p/#ycleavage)--??1D-GlcNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1L-Fuc,p/#zcleavage)--??1D-GlcNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p--??1D-Gal,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??1D-GlcNAc,p--??1D-Gal,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
570.28 49.0
571.39 5.3
572.15 2.5
587.29 1.9

Annotations:

YY redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1L-Fuc,p/#ycleavage)--??1D-GlcNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd YY redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p--??1D-Gal,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
611.29 2.1
625.44 6.2
626.33 3.6
636.29 2.1
637.3 5.4
638.34 4.2
654.33 3.8
655.35 35.2
656.37 7.3
667.18 3.9
667.42 1.8
668.46 3.1
673.51 14.4
674.5 2.1
679.3 3.5
691.37 1.6
697.35 29.8
698.43 6.1
707.31 9.6
708.27 4.7
709.42 2.2
713.46 4.2

Annotations:

ZZ redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1L-Fuc,p/#zcleavage)--??1D-GlcNAc,p--??1D-Gal,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
714.2 5.3
715.36 571.8

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1L-Fuc,p)--??1D-GlcNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??1D-GlcNAc,p--??1D-Gal,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
716.36 125.5
733.35 36.5

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1L-Fuc,p)--??1D-GlcNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p--??1D-Gal,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
734.41 10.4
739.42 1.6
749.35 7.8

Annotations:

YY redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1L-Fuc,p/#ycleavage)--??1D-GlcNAc,p--??1D-Gal,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
750.37 2.1
757.41 7.8
817.38 4.2
818.44 1.7
835.42 3.7
836.38 4.2
837.33 1.9
841.36 1.7
861.36 2.6
877.56 12.2

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1L-Fuc,p)--??1D-GlcNAc,p--??1D-Gal,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1L-Fuc,p/#zcleavage)--??1D-GlcNAc,p--??1D-Gal,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
878.53 8.7
879.52 2.5
880.26 1.6
895.42 69.0

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1L-Fuc,p)--??1D-GlcNAc,p--??1D-Gal,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1L-Fuc,p/#ycleavage)--??1D-GlcNAc,p--??1D-Gal,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
896.42 48.5
897.09 2.4
897.42 4.8
920.65 1.8
921.52 1.4
938.0 1.6
943.45 5.8
944.14 1.9
962.31 1.9
963.53 3.1
964.22 2.6
980.41 1.9
981.48 94.3
982.47 44.7
996.2 1.7
997.44 1.9
999.46 49.4
1000.45 36.9
1001.59 3.8
1001.99 1.7
1005.19 1.4
1005.51 1.9
1006.35 2.7
1010.27 1.8
1011.91 2.8

There are 17 similar spectra:

Id Structure Score

7656

1.00

1.00

5806

0.98

7657

0.98

7673

0.98

0.98

5597

0.97

4878

0.97

0.97

5524

0.96

4251

0.96

5243

0.96

7241

0.91

4270

0.70

3896

0.69

7240

0.61

5595

0.54

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1040.41
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

N/A

MIRAGE Structural data

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