Sulfation of O-glycans on mucin-type proteins from serous ovarian epithelial tumors

Kristina A. Thomsson, Varvara Vitiazeva, Constantina Mateoiu, Chunsheng Jin, Jining Liu, Jan Holgersson, Karin Sundfeldt, Niclas G. Karlsson, Manuscript

Retention Time Recorded Precursor Mass Structure
20.7 829.24 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--6?1S)--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
25.29 559.71 [M -2H]2- redEnd--??1D-GalNAc,p(--3b1D-Gal,p(--3a2D-NeuAc,p)--6?1S)--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-2H,0,redEnd
25.29 1120.33 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p(--3a2D-NeuAc,p)--6?1S)--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
32.37 599.64 [M -2H]2- redEnd--??1D-GalNAc,p(--3b1D-Gal,p(--6?1S)--3a2D-NeuAc,p)--6b1D-GlcNAc,p--4b1D-Gal,p--6?1S$MONO,Und,-2H,0,redEnd

Structure Format

SNFG Oxford Text

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE

See report

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