The intestinal O-glycome is modified by reactive oxygen species deficiency

Radka Saldova, Kristina A. Thomsson, Hayden Wilkinson, Maitrayee Chatterjee, Niclas G. Karlsson, Ulla G. Knaus, Manuscript

Retention Time Recorded Precursor Mass Structure
13.08 384.23 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p$MONO,Und,-H,0,redEnd
17.46 667.42 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p--??1S$MONO,Und,-H,0,redEnd
17.91 675.42 [M -1H]1- redEnd--??1D-GalNAc,p--??1D-Gal,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
18.6 749.3 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
19.21 975.53 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p(--??1D-Gal,p--??1L-Fuc,p)--??1S$MONO,Und,-H,0,redEnd
19.77 895.3 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p--3b1D-Gal,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
21.58 1331.56 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p--??2D-NeuAc,p)--??1D-GlcNAc,p(--??1D-Gal,p)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
22.35 895.3 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p--??1D-Gal,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
26.57 530.2 [M -1H]1- redEnd--??1D-GalNAc,p--??1D-Gal,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
27.02 895.3 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1L-Fuc,p)--??1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
27.2 1041.55 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1L-Fuc,p)--??1D-GlcNAc,p--??1D-Gal,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
28.3 1121.61 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1L-Fuc,p)--??1D-GlcNAc,p(--??1D-Gal,p--??1L-Fuc,p)--??1S$MONO,Und,-H,0,redEnd
28.34 813.24 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1L-Fuc,p)--??1D-GlcNAc,p--??1S$MONO,Und,-H,0,redEnd
28.55 1041.55 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1L-Fuc,p)--??1D-GlcNAc,p--??1D-Gal,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
28.6 1186.65 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1L-Fuc,p)--??1D-GlcNAc,p--??1D-Gal,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
29.3 738.26 [M -2H]2- redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1L-Fuc,p)--??1D-GlcNAc,p(--??1D-Gal,p--??2D-NeuAc,p)--??2D-NeuAc,p$MONO,Und,-2H,0,redEnd

Structure Format

SNFG Oxford Text

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

N/A

MIRAGE

See report

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