Structure

    redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd

Gill mucus and gill mucin O-glycosylation in healthy and amebic gill disease affected Atlantic salmon

GlyTouCan Accession Number: Loading...

Peak Intensity
279.18 14.9
297.26 3.2
298.24 2.3
309.17 9.0
312.16 3.5
323.05 2.3
324.36 8.0

Annotations:

^{2,4}X_{Gal} redEnd--??1D-GalNAc,p--3b1D-Gal,p/#xcleavage_2_4$MONO,Und,-H,0,redEnd
330.17 5.4

Annotations:

BZ b1D-Gal,p/#bcleavage--3b1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
330.47 3.9
338.94 9.6
339.27 3.4
342.23 2.4
346.18 12.2
346.95 3.5
348.14 140.9

Annotations:

BY b1D-Gal,p/#bcleavage--3b1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd CZ b1D-Gal,p/#ccleavage--3b1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
350.95 2.1
365.68 3.6
366.33 6.7

Annotations:

CY b1D-Gal,p/#ccleavage--3b1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p--3b1D-Gal,p--3b1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
368.98 3.1
371.04 3.5
384.17 494.5

Annotations:

Y redEnd--??1D-GalNAc,p--3b1D-Gal,p--3b1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
385.34 4.5
387.26 32.6

Annotations:

BZ b1D-Gal,p/#bcleavage--3b1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
405.26 8.8

Annotations:

BY b1D-Gal,p/#bcleavage--3b1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ b1D-Gal,p/#ccleavage--3b1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
407.4 3.3
419.03 2.6
427.1 2.0
434.27 2.1
437.22 2.3
439.2 2.2
461.26 14.1
463.22 8.5
471.28 3.7
473.02 3.0
479.29 64.4
490.81 4.0
491.22 2.8
514.97 5.0
515.32 2.5
521.24 181.1
522.01 3.1
525.21 2.3
527.21 15.0
532.91 7.9
533.43 4.0
545.44 4.4
549.23 2.5

Annotations:

BZ ?1D-GalNAc,p/#bcleavage--3b1D-Gal,p--3b1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
549.99 4.7
551.21 2913.9

Annotations:

B b1D-Gal,p/#bcleavage--3b1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd ZZ redEnd--??1D-GalNAc,p--3b1D-Gal,p--3b1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
552.23 4.5
553.97 2.3
562.34 3.4
566.9 2.3

Annotations:

BY ?1D-GalNAc,p/#bcleavage--3b1D-Gal,p--3b1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--3b1D-Gal,p--3b1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
569.2 169.3

Annotations:

C b1D-Gal,p/#ccleavage--3b1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p--3b1D-Gal,p--3b1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p--3b1D-Gal,p--3b1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
587.35 58.4

Annotations:

YY redEnd--??1D-GalNAc,p--3b1D-Gal,p--3b1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
588.38 22.9
589.48 2.3
591.18 2.0
593.26 2.9
611.2 2.6

Annotations:

^{2,4}A_{Gal} b1D-Gal,p/#acleavage_2_4--3b1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
618.44 2.4
643.3 2.7
644.27 5.5
651.76 4.4
663.9 3.7
664.37 7.8
672.0 2.6
674.27 4.0
674.97 5.8
675.45 13.2
676.46 4.8
681.89 2.4
682.32 8.4
713.44 3.4

Annotations:

B ?1D-GalNAc,p/#bcleavage--3b1D-Gal,p--3b1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
724.44 3.0
736.18 2.2
738.45 2.3
740.32 2.6
745.31 4.9
752.0 6.7
756.49 2.9
757.25 2.5
786.11 3.8
789.79 4.6
790.21 8.7

Annotations:

Y redEnd--??1D-GalNAc,p--3b1D-Gal,p--3b1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
791.29 9.2
792.33 2.2
828.33 6.5
837.38 3.0
858.17 2.0
876.22 29.8
876.57 13.7
878.15 4.0
881.23 2.5
892.0 4.6
894.38 17.1
899.2 10.5
899.93 2.7
900.56 2.9
909.45 3.8

There are 7 similar spectra:

Id Structure Score

5726

1.00

4866

1.00

1.00

6810

1.00

8006

0.99

8008

0.94

8007

0.94

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 935.38
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Salmo Salar
Tissue - Gills

HPLC

Column

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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