Structure

    redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd

Rainbow trout gastrointestinal mucus, mucin production, mucin glycosylation and response to lipopolysaccharide

GlyTouCan Accession Number: Loading...

Peak Intensity
297.22 2.2
318.09 2.3
321.09 2.9
323.9 2.2
324.33 0.6
329.79 1.1
330.31 2.6

Annotations:

BZ b1D-Gal,p/#bcleavage--??1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
337.11 1.1
348.18 20.3

Annotations:

BY b1D-Gal,p/#bcleavage--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd CZ b1D-Gal,p/#ccleavage--??1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
368.97 1.2
384.23 78.9

Annotations:

Y redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
384.66 1.4
387.2 18.9

Annotations:

BZ b1D-Gal,p/#bcleavage--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
387.86 0.0
405.04 4.1

Annotations:

BY b1D-Gal,p/#bcleavage--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ b1D-Gal,p/#ccleavage--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
405.61 0.0
440.29 2.5
443.91 1.7
445.47 1.1
461.31 6.4
462.84 1.0
463.24 0.9
479.33 18.0
479.95 0.0
485.4 1.2
488.35 0.6
489.89 0.9
509.27 4.2
509.84 0.0
521.18 28.6
521.57 1.3
528.23 1.3

Annotations:

CY ?1D-GalNAc,p/#ccleavage--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
532.37 3.0
551.33 453.4

Annotations:

B b1D-Gal,p/#bcleavage--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
552.23 1.2
569.33 8.9

Annotations:

C b1D-Gal,p/#ccleavage--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
569.69 3.1
577.19 1.7
583.37 2.4
587.56 5.7

Annotations:

YY redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
588.12 0.0
589.47 1.0
596.9 1.1
611.37 4.7
611.94 0.0
634.43 1.4
645.29 4.0
655.53 4.3
656.1 0.0
665.35 4.1
665.89 0.0
694.1 1.2
694.51 2.9
697.25 1.3
702.96 4.2
703.52 0.0
717.22 3.3
719.42 2.8
723.28 0.9
743.08 1.9
749.31 1.3
756.49 3.1
757.03 0.0
770.24 10.1
770.81 0.0
773.77 2.6

Annotations:

^{2,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_2_4--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
776.04 3.1
790.3 2.1

Annotations:

Y redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
791.48 1.5
798.17 1.2
808.49 2.9
831.43 3.4
835.22 0.7
836.32 3.6
845.48 0.8
853.44 3.7
853.99 0.0
855.59 2.7
855.9 1.5
861.48 3.3
862.03 0.0
867.59 1.9
872.14 2.7
873.4 2.8
873.74 2.1
876.28 1.7
886.3 2.5
893.37 1.4
894.3 1.3
897.67 5.3
898.22 0.0
899.18 0.7
899.6 1.5
900.14 4.4
900.7 0.0
904.91 2.4
905.24 3.3
905.79 0.0

There are 7 similar spectra:

Id Structure Score

5439

1.00

4866

1.00

1.00

5726

1.00

8006

0.99

8008

0.94

8007

0.93

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 935.38
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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