Structure

    redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd

Gill mucus and gill mucin O-glycosylation in healthy and amebic gill disease affected Atlantic salmon

GlyTouCan Accession Number: Loading...

Peak Intensity
342.1 1.4
358.99 1.1
364.06 2.2

Annotations:

BY ?1D-GalNAc,p/#bcleavage--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
365.27 2.8

Annotations:

^{2,4}X_{GalNAc} redEnd--??1D-GalNAc,p/#xcleavage_2_4--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
381.6 0.9
382.11 2.6

Annotations:

CY ?1D-GalNAc,p/#ccleavage--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
384.12 1.1

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
387.18 3.0

Annotations:

BZ b1D-Gal,p/#bcleavage--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
389.1 1.1
403.93 1.2
404.74 1.4
405.1 9.5

Annotations:

BY b1D-Gal,p/#bcleavage--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ b1D-Gal,p/#ccleavage--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
405.42 1.7
407.18 124.1

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
425.33 14.2

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
473.06 1.0
508.99 1.0

Annotations:

^{0,4}X_{GalNAc}Z redEnd--??1D-GalNAc,p/#xcleavage_0_4--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
527.32 18.1

Annotations:

^{0,4}X_{GalNAc}Y redEnd--??1D-GalNAc,p/#xcleavage_0_4--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
545.35 2.9
549.25 1.5

Annotations:

BZ ?1D-GalNAc,p/#bcleavage--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
550.07 1.6
551.16 30.8

Annotations:

B b1D-Gal,p/#bcleavage--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
567.18 3.2

Annotations:

BY ?1D-GalNAc,p/#bcleavage--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
568.5 2.1
569.06 2.0

Annotations:

C b1D-Gal,p/#ccleavage--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
569.37 2.0
574.3 1.0
587.24 317.1

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
588.34 1.0
610.23 2.5
625.04 1.1
628.33 1.1
635.24 1.3
646.43 1.4
662.12 1.1
664.42 7.7
670.37 2.5
675.21 1.2
681.99 6.7
682.38 12.4
687.98 2.4
688.19 1.8
714.33 1.1
724.28 24.0
730.31 73.2

Annotations:

^{0,4}X_{GalNAc}Y redEnd--??1D-GalNAc,p/#xcleavage_0_4--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
747.16 0.9
748.42 1.4
752.25 1.3
753.38 1.9
754.32 3.6
771.91 3.8
772.39 15.1

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
790.3 164.8

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
791.04 1.1
791.54 1.2
807.39 1.7
825.46 6.4
827.7 1.5
828.26 1.8
838.28 1.2
867.48 9.4
868.39 1.0
873.53 1.0
876.22 1.6

Annotations:

^{0,4}X_{GalNAc} redEnd--??1D-GalNAc,p/#xcleavage_0_4--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
879.25 0.9
885.05 6.3
885.41 18.4
898.55 1.4
908.66 1.6
914.16 1.1
915.32 4.8
918.1 2.3
918.56 2.6

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
927.33 42.4
936.12 3.6

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
936.52 2.9
939.44 2.0
956.26 3.0
957.23 1.2
959.46 1.0
975.44 8.8

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
975.65 3.5
982.4 1.1
987.83 1.3
989.2 1.2
993.33 3.7

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
994.33 3.2
1019.53 2.0
1031.12 1.1
1037.28 1.0
1040.18 2.3
1059.94 1.4
1061.41 1.9
1079.38 62.2
1084.1 1.8
1090.17 1.7
1096.98 1.1
1097.42 6.3
1111.18 3.4
1111.98 1.6

There are 13 similar spectra:

Id Structure Score

5459

0.94

5749

0.90

5455

0.89

4891

0.85

4892

0.84

4817

0.82

5456

0.81

5750

0.81

4893

0.77

5457

0.65

5460

0.55

7176

0.53

4890

0.50

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1138.45
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Salmo Salar
Tissue - Gills

HPLC

Column

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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