Structure

    redEnd--??1D-GalNAc,p--??1D-GalNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd

Rainbow trout mucus O-glycosylation

GlyTouCan Accession Number: Loading...

Peak Intensity
165.93 0.4
166.49 0.0
178.83 5.6

Annotations:

C ?1D-GalNAc,p/#ccleavage--??1D-Gal,p$MONO,Und,-H,0,redEnd
179.61 0.0

Annotations:

C ?1D-GalNAc,p/#ccleavage--??1D-Gal,p$MONO,Und,-H,0,redEnd
179.99 0.8

Annotations:

C ?1D-GalNAc,p/#ccleavage--??1D-Gal,p$MONO,Und,-H,0,redEnd
180.54 0.0
201.34 2.3

Annotations:

BY ?1D-GalNAc,p/#bcleavage--??1D-GalNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--??1D-GalNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
201.98 9.3

Annotations:

BY ?1D-GalNAc,p/#bcleavage--??1D-GalNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--??1D-GalNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
202.63 0.0

Annotations:

BY ?1D-GalNAc,p/#bcleavage--??1D-GalNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--??1D-GalNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
204.25 1.5

Annotations:

Z redEnd--??1D-GalNAc,p--??1D-GalNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
204.82 0.0

Annotations:

Z redEnd--??1D-GalNAc,p--??1D-GalNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
205.27 0.3
221.88 3.1

Annotations:

Y redEnd--??1D-GalNAc,p--??1D-GalNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
222.22 1.1

Annotations:

Y redEnd--??1D-GalNAc,p--??1D-GalNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
222.78 0.0

Annotations:

Y redEnd--??1D-GalNAc,p--??1D-GalNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
298.93 0.6
299.21 1.1
299.48 0.0
299.77 0.0
316.72 1.4
317.28 0.0
318.99 1.0
319.54 0.0
347.04 6.3
347.24 4.2
347.93 0.9
348.49 0.0
359.22 3.3
359.77 0.0
364.12 7.9

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1D-GalNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
365.02 2.2

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1D-GalNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
365.57 0.0
366.26 2.8
366.78 0.0
366.99 0.0
370.19 0.5
375.47 2.8
376.02 0.0
377.06 1.2
377.61 0.0
390.47 1.6
391.03 0.0
406.65 2.6

Annotations:

Z redEnd--??1D-GalNAc,p--??1D-GalNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
407.18 2.7

Annotations:

Z redEnd--??1D-GalNAc,p--??1D-GalNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
407.71 0.0

Annotations:

Z redEnd--??1D-GalNAc,p--??1D-GalNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
408.37 0.6
408.94 0.0
414.72 0.8
415.28 0.0
424.15 3.1
424.7 0.0

Annotations:

Y redEnd--??1D-GalNAc,p--??1D-GalNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
425.26 3.4

Annotations:

Y redEnd--??1D-GalNAc,p--??1D-GalNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
425.97 2.3

Annotations:

Y redEnd--??1D-GalNAc,p--??1D-GalNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
426.58 1.4
427.13 0.0
436.98 0.5
437.4 0.6
438.09 2.3
438.63 0.0
455.22 0.9
455.78 0.0
467.13 1.2
467.69 0.0
470.08 1.8
470.62 0.0
471.27 1.3
471.81 0.0
480.08 1.8
480.62 0.0
485.61 0.8
486.19 0.0
488.91 0.4
489.46 0.0
490.05 0.3
511.07 0.7
511.62 0.0
511.91 0.4
512.46 0.0
513.54 0.9
514.11 0.0
515.08 0.4
515.64 0.0
526.3 1.5
527.29 71.3
528.42 13.8
529.06 0.0
530.02 0.5
530.6 0.0
542.11 1.5
542.41 2.0
542.96 0.0
546.17 0.9
546.72 0.0
550.8 0.3
557.27 2.0
557.83 0.0
561.07 0.9
561.62 0.0
630.64 0.4
631.2 0.0

There are 9 similar spectra:

Id Structure Score

7390

0.88

7419

0.88

5508

0.61

4800

0.58

7139

0.58

3970

0.52

3741

0.52

4229

0.51

5221

0.51

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 586.24
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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