Structure

    redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??2D-NeuAc,p)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd

Rainbow trout mucus O-glycosylation

GlyTouCan Accession Number: Loading...

Peak Intensity
347.05 0.7

Annotations:

BZ ?1D-GalNAc,p/#bcleavage--??1HexNAc,p--??1D-Gal,p--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
347.61 0.0
387.16 0.3
387.7 0.0
432.13 0.3
432.69 0.0
555.29 0.6
555.86 0.0
570.21 0.5

Annotations:

YZ redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??2D-NeuAc,p/#ycleavage)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??2D-NeuAc,p/#zcleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
570.77 0.0
579.27 1.1
579.81 0.0
580.36 0.0
580.97 1.6
581.35 2.4
581.92 0.0
582.45 0.0
587.02 3.1

Annotations:

YY redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??2D-NeuAc,p/#ycleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
587.44 2.3

Annotations:

YY redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??2D-NeuAc,p/#ycleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
588.29 1.5
588.86 0.0
589.38 0.0
656.17 0.6

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1HexNAc,p--??1D-Gal,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
656.71 0.0
674.44 0.8
675.02 0.0
675.53 0.0
676.37 0.9
676.94 0.0
677.45 0.0
680.4 0.2
680.95 0.0
685.3 0.9
686.03 0.4
686.6 0.0
700.32 0.3
700.87 0.0
716.23 1.3

Annotations:

Y redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
716.52 3.2

Annotations:

Y redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
717.38 1.7
717.92 0.0
718.29 0.4
718.79 0.0
719.35 0.0
726.44 0.5
726.97 0.0
739.49 0.3
740.03 0.0
768.69 0.6
769.22 0.5
769.78 0.0
781.15 1.5
781.71 0.0
782.27 0.0
786.38 1.6
786.94 0.0
787.46 0.0
800.36 4.2
800.99 0.0
801.49 1.2
802.03 0.0
802.57 0.0
816.84 0.8
817.39 0.0
817.95 0.5
818.36 6.9
818.93 0.0
819.38 0.9
819.94 0.0
820.46 0.0
841.67 1.0
842.21 0.0
842.77 0.0
858.08 4.3
858.54 3.8
858.92 0.4
859.46 0.6

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??2D-NeuAc,p)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??2D-NeuAc,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
860.27 56.7

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??2D-NeuAc,p)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??2D-NeuAc,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
860.61 21.0

Annotations:

Z redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??2D-NeuAc,p)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??2D-NeuAc,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
861.41 11.8
861.99 0.0
862.49 0.0
875.37 0.3
876.43 90.3
876.96 11.6
877.38 39.8

Annotations:

Y redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??2D-NeuAc,p)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??2D-NeuAc,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
878.41 2817.4

Annotations:

Y redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??2D-NeuAc,p)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??2D-NeuAc,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
879.44 490.6
880.31 3.3
880.51 2.5
881.32 1.6
881.87 0.0
882.41 0.0
907.24 1.1
907.79 0.0
930.22 1.3
930.78 0.0
931.32 0.0
938.82 0.4
939.37 0.0

There are 4 similar spectra:

Id Structure Score

4897

0.99

7232

0.98

4895

0.98

4896

0.98

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1168.43
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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