Core-2 O-glycans are required for galectin-3 interaction with the synovial glycoprotein lubricin

Sarah A. Flowers, Kristina A. Thomsson, Liaqat Ali, Shan Huang, Yolanda Mthembu, Suresh C. Regmi, Jan Holgersson, Tannin A. Schmidt, Ola Rolfson, Lena I Björkman, Martina Sundqvist, Anna Karlsson, Gregory D. Jay, Thomas Eisler, Roman Krawetz, Niclas G. Karlsson, Manuscript

Retention Time Recorded Precursor Mass Structure
11.25 384.0 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p$MONO,Und,-H,0,redEnd
15.38 425.08 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
15.38 587.04 [M -1H]1- redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
15.47 464.0 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--??1S$MONO,Und,-H,0,redEnd
15.76 790.17 [M -1H]1- redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p--??1HexNAc,p)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
16.33 749.08 [M -1H]1- redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p--4b1D-Gal,p)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
16.43 667.0 [M -1H]1- redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p--??1S)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
17.01 675.25 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
20.36 829.0 [M -1H]1- redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p(--??1D-Gal,p)--??1S)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
20.76 878.19 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-GlcNAc,p)--??1D-Gal,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
20.86 966.17 [M -1H]1- redEnd--??1D-GalNAc,p(--??2D-NeuAc,p)--??1D-Gal,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
21.26 1081.15 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-GlcNAc,p--??1HexNAc,p)--3b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
23.48 1040.25 [M -1H]1- redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p--??1D-Gal,p)--3b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
26.27 958.17 [M -1H]1- redEnd--??1D-GalNAc,p(--?b1D-GlcNAc,p--??1S)--3b1D-Gal,p--3b2D-NeuAc,p$MONO,Und,-H,0,redEnd
27.01 1040.15 [M -1H]1- redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p--??1D-Gal,p--3a2D-NeuAc,p)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
27.75 1119.99 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-GlcNAc,p(--??1D-Gal,p--??2D-NeuAc,p)--??1S)--??1D-Gal,p$MONO,Und,-H,0,redEnd
28.64 1120.02 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-GlcNAc,p(--??1D-Gal,p)--??1S)--??1D-Gal,p}--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
28.96 1405.24 [M -1H]1- redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-Gal,p)--3b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
31.67 1331.17 [M -1H]1- redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p--??1D-Gal,p--3b2D-NeuAc,p)--3b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
32.18 1405.24 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-Gal,p)--??1D-Gal,p}--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
33.01 884.67 [M -2H]2- redEnd--??1D-GalNAc,p(--??1D-Gal,p--??2D-NeuAc,p)--??1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-2H,0,redEnd
34.79 705.11 [M -2H]2- redEnd--??1D-GalNAc,p(--??1D-GlcNAc,p(--??1D-Gal,p)--??1S)--??1D-Gal,p}(--??2D-NeuAc,p)--??2D-NeuAc,p$MONO,Und,-2H,0,redEnd
36.19 884.75 [M -2H]2- redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-Gal,p--??2D-NeuAc,p$MONO,Und,-2H,0,redEnd
36.39 847.71 [M -2H]2- redEnd--??1D-GalNAc,p(--??1D-GlcNAc,p--??1D-Gal,p--??1D-GlcNAc,p--??1D-Gal,p--??2D-NeuAc,p)--??1D-Gal,p--??2D-NeuAc,p$MONO,Und,-2H,0,redEnd

Structure Format

SNFG Oxford Text

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE

See report

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