Structure

    redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p)--3b1D-Gal,p$MONO,Und,-H,0,redEnd

Core-2 O-glycans are required for galectin-3 interaction with the synovial glycoprotein lubricin

GlyTouCan Accession Number: Loading...

Peak Intensity
173.02 0.8
173.6 0.0
174.11 0.0
174.66 0.0
175.2 0.0
175.73 0.0
176.28 0.0
176.8 0.0
177.36 0.0
177.87 0.0
179.05 1.5

Annotations:

C ?1D-GalNAc,p/#ccleavage--3b1D-Gal,p$MONO,Und,-H,0,redEnd
179.64 0.0
180.19 0.0
180.72 0.0
181.27 0.0
181.79 0.0
182.36 0.0
182.87 0.0
183.44 0.0
183.94 0.0
185.78 1.0

Annotations:

ZZ redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p/#zcleavage)--3b1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
186.12 3.0

Annotations:

ZZ redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p/#zcleavage)--3b1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
186.7 0.0
187.24 0.0
187.78 0.0
188.31 0.0
188.86 0.0
189.38 0.0
189.94 0.0
190.46 0.0
191.02 0.0
191.69 1.1
192.02 2.9
192.59 0.0
193.11 0.0
193.67 0.0
194.19 0.0
194.76 0.0
195.27 0.0
195.8 0.0
196.35 0.0
196.87 0.0
198.13 1.2
198.69 0.0
199.21 0.0
199.77 0.0
200.29 0.0
200.78 0.3
201.17 0.3
201.95 11.1

Annotations:

B ?1D-GalNAc,p/#bcleavage--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
202.67 0.0
203.22 1.1
204.08 16.3

Annotations:

ZY redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p/#zcleavage)--3b1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p/#ycleavage)--3b1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
204.87 0.0
205.33 0.0
206.22 1.2
206.78 0.0
207.31 0.0
207.86 0.0
208.39 0.0
208.89 0.3
209.45 0.0
210.01 0.0
210.53 0.0
211.07 0.0
211.61 0.0
212.14 0.0
212.69 0.0
213.22 0.0
214.91 0.7
215.46 0.0
216.02 0.0
216.53 0.0
217.1 0.0
217.83 2.2
218.13 0.7
218.95 1.5
219.85 3.2

Annotations:

C ?1D-GalNAc,p/#ccleavage--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
220.43 0.0

Annotations:

C ?1D-GalNAc,p/#ccleavage--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
220.96 0.0

Annotations:

^{2,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_2_4--3b1D-Gal,p$MONO,Und,-H,0,redEnd
221.51 0.0

Annotations:

^{2,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_2_4--3b1D-Gal,p$MONO,Und,-H,0,redEnd
222.21 2.9

Annotations:

YY redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p/#ycleavage)--3b1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
222.77 0.0
223.3 0.0
223.89 0.0
224.38 0.0
224.98 0.0
225.46 0.0
226.06 0.0
226.54 0.0
226.87 0.5
227.44 0.0
227.96 0.0
228.52 0.0
229.04 0.0
229.6 0.0
230.12 0.0
230.66 0.0
231.2 0.0
241.29 1.9
241.86 0.0
242.37 0.0
242.94 0.0
243.45 0.0
244.01 0.0
244.53 0.0
245.07 0.0
245.61 0.0
245.97 0.5
246.53 0.0
247.05 0.0
247.61 0.0
248.13 0.0
248.69 0.0
249.21 0.0
249.77 0.0
250.28 0.0
251.15 0.6
251.7 0.0
252.27 0.0
252.78 0.0
253.33 0.0
253.86 0.0
254.4 0.0
254.94 0.0
255.47 0.0
256.02 0.0
256.77 0.4
257.33 0.0
257.86 0.0
258.4 0.0
258.94 0.0
259.47 0.0
260.02 0.0
260.55 0.0
261.11 0.0
262.05 8.3

Annotations:

^{0,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_4--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd ^{1,3}A_{GalNAc} ?1D-GalNAc,p/#acleavage_1_3--3b1D-Gal,p$MONO,Und,-H,0,redEnd
262.61 0.0
262.84 0.0
263.33 0.0
263.69 0.0
263.92 0.0
264.42 0.0
264.78 0.0
264.98 0.0
265.5 0.0
265.87 0.0
266.06 0.0
266.58 0.0
266.97 0.0
272.94 1.8
273.26 2.5
273.82 0.0
274.37 0.0
274.89 0.0
275.45 0.0
276.02 3.7

Annotations:

^{3,5}A_{GalNAc} ?1D-GalNAc,p/#acleavage_3_5--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
276.6 0.0

Annotations:

^{3,5}A_{GalNAc} ?1D-GalNAc,p/#acleavage_3_5--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
277.12 0.0
277.68 0.0
278.2 0.0
278.75 0.0
279.28 0.0
279.82 0.0
280.36 0.0
280.88 0.0
285.68 0.4
286.24 0.0
286.78 0.0
287.31 0.0
288.03 0.5
288.38 0.0
288.6 0.0
289.12 0.0
289.46 0.0
289.69 0.0
290.2 0.0
290.75 0.0
291.28 0.0
291.82 0.0

Annotations:

^{0,3}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_3--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd ^{1,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_1_4--3b1D-Gal,p$MONO,Und,-H,0,redEnd
292.36 0.0

Annotations:

^{0,3}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_3--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd ^{1,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_1_4--3b1D-Gal,p$MONO,Und,-H,0,redEnd
298.99 1.4
299.82 1.0
300.37 0.0
300.94 0.0
301.45 0.0
301.99 0.0
302.53 0.0
303.06 0.0
303.61 0.0
304.13 0.0
304.69 0.0
311.98 3.0
312.66 0.0
313.03 0.0
313.25 0.0
313.75 0.0
314.11 0.0
314.33 0.0
314.83 0.0
315.2 0.0
315.39 0.0
315.8 0.4
316.36 0.0
316.82 0.3
317.37 0.0
317.93 0.0
318.45 0.0
318.99 0.0
319.53 0.0
320.06 0.0
320.61 0.0
321.13 0.0
321.89 0.5
322.45 0.0
322.99 0.0
323.53 0.0
324.06 0.0
324.61 0.0
325.13 0.0
325.69 0.0
326.21 0.0
328.63 0.6
328.98 2.1
329.53 0.0
330.07 0.0
330.61 0.0
331.14 0.0
331.69 0.0
332.22 0.0
332.77 0.0
333.29 0.0
333.85 0.0
339.3 0.9
339.86 0.0
340.39 0.0
340.94 0.0
341.46 0.0
342.02 0.0
342.54 0.0
343.29 0.9
343.99 9.5
344.56 0.0
345.1 0.0
345.64 0.0
346.18 0.0
347.13 9.3
347.98 0.5
348.53 0.0
349.08 0.0
349.92 2.4
350.48 0.0
351.0 0.0
351.56 0.0
352.08 0.0
352.64 0.0
353.15 0.0
353.71 0.0
354.24 0.0
354.78 0.0
357.03 1.1
357.61 0.0
358.12 0.0
358.74 2.3
359.04 2.6
359.22 1.2
359.76 0.0
360.26 0.0
360.84 0.0
361.34 0.0
361.92 0.0
362.42 0.0
362.8 0.0
363.0 0.0
363.56 0.8
364.1 2.5
365.09 10.6
365.92 4.6

Annotations:

Z redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p/#zcleavage)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
366.59 0.0

Annotations:

Z redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p/#zcleavage)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
367.09 0.0
367.67 0.0
368.17 0.0
368.75 0.0
369.26 0.0
369.84 0.0
370.34 0.0
370.89 0.8
371.24 0.5
371.82 0.3
372.37 0.0
372.68 0.4
373.23 0.0
373.78 0.0
374.3 0.0
374.86 0.0
375.38 0.0
375.94 0.0
376.45 0.0
377.14 0.7
377.7 0.0
378.23 0.0
378.78 0.0
379.3 0.0
379.86 0.0
380.38 0.0
380.94 0.0
381.46 0.0
382.01 2.0
382.7 0.2
382.97 1.3
384.01 1.6

Annotations:

Y redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p/#ycleavage)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
384.34 0.0

Annotations:

Y redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p/#ycleavage)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
384.6 0.0

Annotations:

Y redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p/#ycleavage)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
385.11 0.0
385.43 0.0
385.67 0.0
386.38 1.2
386.94 0.0
387.46 0.0
388.02 0.0
388.65 1.7
388.92 3.4
389.19 2.1
389.77 0.0
390.4 0.0
390.84 0.0
391.48 0.0
391.92 0.0
392.57 0.0
393.0 0.0
393.65 0.0
394.07 0.0
394.99 0.4
395.54 0.0
396.1 0.0
396.62 0.0
397.17 0.0
397.7 0.0
398.23 0.0
398.78 0.0
399.31 0.0
405.98 0.8
407.0 758.7

Annotations:

Z redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p)--3b1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
407.79 7.2
408.1 15.7
408.76 0.0
409.36 1.0
409.94 0.0
410.45 0.0
410.94 1.6
411.49 0.0
412.03 0.0
412.56 0.0
413.11 0.0
413.63 0.0
414.19 0.0
414.71 0.0
415.27 0.0
415.78 0.0
423.94 0.7
425.04 32.7

Annotations:

Y redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p)--3b1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
425.63 1.3

Annotations:

Y redEnd--??1D-GalNAc,p(--6b1D-GlcNAc,p)--3b1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
426.23 3.4
426.8 0.0
427.37 0.0
427.88 0.0
428.45 0.0
429.18 1.1
429.73 0.0
430.28 0.0
430.8 0.0
431.36 0.0
431.88 0.0
432.44 0.0
432.96 0.0
433.52 0.0
434.03 0.0
442.0 0.9
442.56 0.0
443.11 0.0
443.63 0.0
444.19 0.0
444.71 0.0
445.27 0.0
445.79 0.0
446.35 0.0
446.86 0.0
453.09 1.1
453.64 0.0
454.19 0.0
454.71 0.0
455.27 0.0
455.79 0.0
456.35 0.0
456.87 0.0
457.44 0.0
457.94 0.0
466.95 1.8
467.47 0.5
468.03 1.4

Annotations:

^{2,5}A_{GalNAc} ?1D-GalNAc,p/#acleavage_2_5(--6b1D-GlcNAc,p)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
468.24 0.6

Annotations:

^{2,5}A_{GalNAc} ?1D-GalNAc,p/#acleavage_2_5(--6b1D-GlcNAc,p)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
468.77 0.0
469.11 0.0
469.35 0.0
469.85 0.0
470.19 0.0
470.43 0.0
470.93 0.0
471.27 0.0
471.51 0.0
472.02 0.0
472.37 0.0
472.58 0.0
473.11 0.0
478.75 0.7
479.11 0.3
479.69 0.0
480.2 0.0
480.77 0.0
481.28 0.0
481.83 0.0
482.36 0.0
482.9 0.0
483.44 0.0
483.96 0.0

Annotations:

^{0,2}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_2(--6b1D-GlcNAc,p)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
484.63 0.4

Annotations:

^{0,2}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_2(--6b1D-GlcNAc,p)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
485.04 1.0
485.61 0.0
486.18 0.0
486.68 0.0
487.27 0.0
487.78 0.0
488.35 0.0
488.86 0.0
489.43 0.0
489.92 0.6
490.46 0.0
491.02 0.0
491.54 0.0
492.1 0.0
492.62 0.0
493.18 0.0
493.84 1.1
494.38 0.0
494.94 0.0
495.46 0.0
496.02 0.0
496.53 0.0
497.09 0.0
497.61 0.0
498.15 0.0
498.69 0.0
509.07 0.8
509.62 0.0
510.17 0.0
510.7 0.0
511.23 0.0
511.78 0.0
512.31 0.0
512.86 0.0
513.38 0.0
513.94 0.0
522.41 1.2
522.95 0.0
523.52 0.0
524.03 0.0
524.57 0.0
525.03 0.5
525.6 0.0
526.12 0.0
526.97 68.6
528.24 2.3
528.81 0.0
529.34 0.0
529.81 0.0
530.42 0.0
530.93 0.0
531.5 0.0
532.01 0.0
532.59 0.0
533.08 0.0
539.24 1.5

Annotations:

^{1,5}A_{GalNAc} ?1D-GalNAc,p/#acleavage_1_5(--6b1D-GlcNAc,p)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
539.56 0.6

Annotations:

^{1,5}A_{GalNAc} ?1D-GalNAc,p/#acleavage_1_5(--6b1D-GlcNAc,p)--3b1D-Gal,p$MONO,Und,-H,0,redEnd
540.12 0.0
540.67 0.0
541.2 0.0
541.73 0.0
542.28 0.0
543.04 0.7
543.61 0.0
543.99 0.3
545.12 6.8
545.82 0.0
546.31 0.0
546.85 0.0
547.42 0.0
547.93 0.0
548.5 0.0
549.01 0.0
549.58 0.0
550.09 0.0
554.83 1.3
555.37 0.0
555.98 1.1
556.53 0.0
557.05 0.0
557.61 0.0
558.13 0.0
558.7 5.5
558.93 1.0
559.52 0.0
560.02 0.0
560.61 0.0
561.1 0.0
561.69 0.0
562.19 0.0
562.77 0.0
563.27 0.0

There are 23 similar spectra:

Id Structure Score

1.00

1.00

4833

1.00

4259

1.00

0.99

5415

0.99

3110

0.99

4077

0.99

7976

0.98

3998

0.97

5416

0.89

0.87

4800

0.87

7876

0.81

0.78

7680

0.78

7687

0.78

5694

0.71

4231

0.62

5223

0.62

4229

0.62

5221

0.62

3743

0.61

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 586.24
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

Report errors in this entry

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