Stress impairs skin barrier function and induces α2-3 linked N-acetylneuraminic acid and core 1 O-glycans on skin mucins in Atlantic salmon, Salmo salar

John Benktander, Henrik Sundh, Kristina Sundell, Abarna V. M. Murugan, Vignesh Venkatakrishnan, János Tamás Padra, Jelena Kolarevic, Bendik Fyhn Terjesen, Marnix Gorissen and Sara K. Lindén, Manuscript

Retention Time Recorded Precursor Mass Structure
5.08 472.19 [M -1H]1- redEnd--??1D-GalNAc,p--6a2D-KDN,p$MONO,Und,-H,0,redEnd
7.48 384.16 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p$MONO,Und,-H,0,redEnd
7.94 425.17 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
8.52 733.24 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-GlcNAc,p(--4b1D-Gal,p)--3a1L-Fuc,p$MONO,Und,-H,0,redEnd
8.82 634.29 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6a2D-KDN,p$MONO,Und,-H,0,redEnd
8.9 529.28 [M -1H]1- redEnd--??1D-GalNAc,p--6a2D-NeuGc,p$MONO,Und,-H,0,redEnd
9.06 513.2 [M -1H]1- redEnd--??1D-GalNAc,p--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
9.47 587.1 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
9.47 691.17 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6a2D-NeuGc,p$MONO,Und,-H,0,redEnd
9.55 675.22 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
9.72 593.09 [M -1H]1- redEnd--??1D-GalNAc,p--6a2D-NeuAc,p--??1S$MONO,Und,-H,0,redEnd
9.74 587.1 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
9.92 425.17 [M -1H]1- redEnd--??1D-GalNAc,p--3a1D-GalNAc,p$MONO,Und,-H,0,redEnd
10.01 675.22 [M -1H]1- redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p)--6a2D-KDN,p$MONO,Und,-H,0,redEnd
10.23 732.39 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-GlcNAc,p)--6a2D-NeuGc,p$MONO,Und,-H,0,redEnd
10.27 716.35 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-GlcNAc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
10.43 593.09 [M -1H]1- redEnd--??1D-GalNAc,p(--6a2D-NeuAc,p)--??1S$MONO,Und,-H,0,redEnd
10.53 587.1 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
10.7 790.19 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
10.7 1098.25 [M -1H]1- redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1D-GlcNAc,p(--??1L-Fuc,p)--??1D-Gal,p$MONO,Und,-H,0,redEnd
10.84 571.18 [M -1H]1- redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
10.92 691.17 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p--3a2D-NeuGc,p$MONO,Und,-H,0,redEnd
11.18 755.12 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6a2D-NeuAc,p}--??1S$MONO,Und,-H,0,redEnd
11.2 790.29 [M -1H]1- redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3?1D-Gal,p)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
11.26 675.22 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
11.34 749.17 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
11.53 571.18 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-GlcNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
11.53 790.23 [M -1H]1- redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
11.6 755.17 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6a2D-NeuAc,p}--??1S$MONO,Und,-H,0,redEnd
11.7 733.23 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
11.98 749.17 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p--3b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
12.02 894.28 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-GlcNAc,p--4b1D-Gal,p)--6a2D-NeuGc,p$MONO,Und,-H,0,redEnd
12.07 966.08 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
12.11 732.39 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-GalNAc,p)--6a2D-NeuGc,p$MONO,Und,-H,0,redEnd
12.2 878.24 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-GlcNAc,p--4b1D-Gal,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
12.24 733.23 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
12.25 716.35 [M -1H]1- redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
12.46 894.28 [M -1H]1- redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1D-Gal,p)--6a2D-NeuGc,p$MONO,Und,-H,0,redEnd
12.52 878.22 [M -1H]1- redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--4?1D-Gal,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
12.56 952.33 [M -1H]1- redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--3?1D-Gal,p--4?1HexNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
12.79 936.19 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
12.84 952.33 [M -1H]1- redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
13.16 936.17 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
13.17 790.24 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
13.25 862.27 [M -1H]1- redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1L-Fuc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
13.36 936.19 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
13.44 936.17 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--4?1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
13.45 1081.34 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
13.61 1024.29 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
13.65 1040.43 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6a2D-NeuGc,p$MONO,Und,-H,0,redEnd
13.72 878.22 [M -1H]1- redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1L-Fuc,p)--6a2D-NeuGc,p$MONO,Und,-H,0,redEnd
14.19 1139.3 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
14.26 1024.29 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
14.27 936.17 [M -1H]1- redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
14.27 1227.34 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--3b1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
14.56 790.23 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-GlcNAc,p--4b1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
14.67 1139.3 [M -1H]1- redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--3b1D-Gal,p--3?1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
14.72 1259.37 [M -1H]1- redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--??1D-Gal,p)--6a2D-NeuGc,p$MONO,Und,-H,0,redEnd
15.0 1243.36 [M -1H]1- redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--4b1D-Gal,p--??1HexNAc,p--??1D-Gal,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
15.29 1243.37 [M -1H]1- redEnd--??1D-GalNAc,p(--3?1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6a2D-NeuGc,p$MONO,Und,-H,0,redEnd
15.33 1301.35 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1L-Fuc,p)--??1HexNAc,p)--6b1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
15.37 1114.28 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1D-Gal,p)--6b1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
15.47 1098.29 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
15.48 1227.34 [M -1H]1- redEnd--??1D-GalNAc,p(--3?1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b2D-NeuAc,p$MONO,Und,-H,0,redEnd
15.51 1257.25 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6a2D-NeuAc,p--8a2D-NeuAc,p$MONO,Und,-H,0,redEnd
15.65 1098.25 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--3?1D-GlcNAc,p(--??1L-Fuc,p)--??1D-Gal,p)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
15.69 1040.43 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6b1D-GlcNAc,p--3b1D-Gal,p$MONO,Und,-H,0,redEnd
15.75 1243.36 [M -1H]1- redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuGc,p$MONO,Und,-H,0,redEnd
15.83 751.97 [M -2H]2- redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-2H,0,redEnd
15.85 936.17 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
15.94 1227.34 [M -1H]1- redEnd--??1D-GalNAc,p(--3?1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b2D-NeuAc,p$MONO,Und,-H,0,redEnd
16.55 936.24 [M -1H]1- redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
16.57 1463.49 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--??1D-Gal,p(--3?1D-Gal,p)--6?1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
17.17 1301.35 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1L-Fuc,p)--??1HexNAc,p)--6b1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
17.33 751.95 [M -2H]2- redEnd--??1D-GalNAc,p--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-2H,0,redEnd
17.35 1098.4 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1D-GlcNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
17.77 1301.39 [M -1H]1- redEnd--??1D-GalNAc,p--3?1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
17.85 1098.29 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
18.11 795.97 [M -2H]2- redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p--??1D-Gal,p)--6a2D-NeuAc,p$MONO,Und,-2H,0,redEnd
18.19 1040.43 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
19.19 1040.43 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1D-GlcNAc,p--4b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd

Structure Format

SNFG Oxford Text

Biological Context

Taxonomy - Salmo Salar
Tissue - Mucus

HPLC

Column Hypercarb

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE

See report

UniCarb-DR Creative Commons License

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