Structure

    redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--3a1L-Fuc,p$MONO,Und,-H,0,redEnd

Gill mucus and gill mucin O-glycosylation in healthy and amebic gill disease affected Atlantic salmon

GlyTouCan Accession Number: Loading...

Peak Intensity
161.79 1.6
162.09 1.7
166.05 5.9
172.02 3.7
174.06 0.8
178.13 1.0
183.69 1.4
184.06 13.6

Annotations:

BZ ?1D-GalNAc,p/#bcleavage--3a1D-GalNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
184.69 0.0
186.06 2.2
187.87 3.1
192.19 1.2
194.99 1.3
201.94 325.9

Annotations:

BY ?1D-GalNAc,p/#bcleavage--3a1D-GalNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--3a1D-GalNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
203.02 0.0
204.08 237.9

Annotations:

Z redEnd--??1D-GalNAc,p--3a1D-GalNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
204.97 1.4
208.95 2.3
215.16 1.0
217.02 0.4
219.81 1.5

Annotations:

CY ?1D-GalNAc,p/#ccleavage--3a1D-GalNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
222.12 86.8

Annotations:

Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
222.86 0.0
233.22 2.2
239.14 2.5
257.33 2.8
257.82 1.7
258.95 0.8
275.02 4.1
275.3 0.7
288.02 0.7
290.42 1.2
299.14 44.5
305.07 2.5
306.11 0.8
311.02 14.0
317.2 20.8
317.88 0.0
318.51 1.6
320.93 2.0
329.08 4.1
329.8 1.0
330.25 4.8
330.48 2.2
331.21 1.4
333.14 1.6
339.11 2.7
341.09 4.0
347.21 74.2
348.2 121.7

Annotations:

B ?1D-GalNAc,p/#bcleavage--3a1D-GalNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
349.11 0.0
355.38 2.4
358.94 3.6
365.2 98.5
367.31 0.5
370.98 1.0
388.8 1.8
389.22 0.7
391.27 0.9
395.03 1.4
399.34 1.3
407.2 61.3

Annotations:

Z redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
407.98 0.0
421.19 27.1
425.26 23.0

Annotations:

Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
426.17 2.0
431.98 2.4
442.0 0.8
443.08 2.9
450.16 0.7
450.91 2.6
451.27 11.7
451.95 0.0
453.2 1.8
454.02 1.2
455.03 3.1
463.1 20.5
463.36 14.7
463.68 2.1
464.03 0.9
469.24 2.2
471.26 1.1
473.26 0.7
481.36 1.5
493.12 9.0
495.29 3.7
505.22 0.8
511.22 1176.2

Annotations:

^{0,4}X_{GalNAc} redEnd--??1D-GalNAc,p/#xcleavage_0_4--3a1D-GalNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
512.15 0.0
513.39 1.6
515.86 1.0
522.99 2.5
523.32 3.5
526.15 3.1
529.36 14.9
530.03 0.0
531.05 0.9
535.11 6.6
541.36 2.5
544.61 1.4

There are 17 similar spectra:

Id Structure Score

5691

1.00

0.95

7973

0.95

7974

0.94

0.93

0.93

7975

0.93

4839

0.91

0.91

5414

0.89

4799

0.83

0.83

6794

0.83

7401

0.78

7430

0.78

7136

0.76

5692

0.50

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 570.24
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Salmo Salar
Tissue - Gills

HPLC

Column

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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