Structure

    redEnd--??1D-GalNAc,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd

Rainbow trout mucus O-glycosylation

GlyTouCan Accession Number: Loading...

Peak Intensity
183.76 0.7

Annotations:

BZ ?1D-GalNAc,p/#bcleavage--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
184.31 0.0

Annotations:

BZ ?1D-GalNAc,p/#bcleavage--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
184.86 0.0

Annotations:

BZ ?1D-GalNAc,p/#bcleavage--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
193.13 0.8
193.69 0.0
194.22 0.0
197.91 1.0
198.46 0.0
199.02 0.0
201.94 5.3

Annotations:

BY ?1D-GalNAc,p/#bcleavage--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
202.17 1.5

Annotations:

BY ?1D-GalNAc,p/#bcleavage--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
202.53 0.0

Annotations:

BY ?1D-GalNAc,p/#bcleavage--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
202.76 0.0

Annotations:

BY ?1D-GalNAc,p/#bcleavage--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
203.27 0.0

Annotations:

Z redEnd--??1D-GalNAc,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
203.79 1.9

Annotations:

Z redEnd--??1D-GalNAc,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
204.13 0.8

Annotations:

Z redEnd--??1D-GalNAc,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
204.69 0.0

Annotations:

Z redEnd--??1D-GalNAc,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
205.01 0.7

Annotations:

Z redEnd--??1D-GalNAc,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
205.56 0.0
206.11 0.0
219.56 1.2

Annotations:

CY ?1D-GalNAc,p/#ccleavage--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
220.11 0.0

Annotations:

CY ?1D-GalNAc,p/#ccleavage--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
220.64 0.0

Annotations:

CY ?1D-GalNAc,p/#ccleavage--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
258.03 2.5
258.59 0.0
259.11 0.0
299.07 0.7
299.62 0.0
300.18 0.0
327.91 1.7
328.47 0.0
329.26 0.8
329.81 0.0
330.36 0.0
331.07 2.5
331.62 0.0
332.16 0.0
332.7 0.0
347.1 1.1
347.35 0.9

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
348.03 5.9

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
348.29 2.8

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
348.86 0.0

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
349.4 0.0
364.73 1.3
365.29 0.0

Annotations:

C ?1D-GalNAc,p/#ccleavage--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
365.85 0.0

Annotations:

C ?1D-GalNAc,p/#ccleavage--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
371.27 3.2
371.85 0.0
372.37 0.0
397.85 2.1
398.39 0.0
398.94 0.0
407.07 0.5

Annotations:

Z redEnd--??1D-GalNAc,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
407.62 0.0

Annotations:

Z redEnd--??1D-GalNAc,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
408.16 0.0

Annotations:

Z redEnd--??1D-GalNAc,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
425.05 0.7

Annotations:

Y redEnd--??1D-GalNAc,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
425.61 0.0

Annotations:

Y redEnd--??1D-GalNAc,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
426.01 0.7

Annotations:

Y redEnd--??1D-GalNAc,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
426.56 0.0
427.11 0.0
463.25 1.4
463.79 0.0
464.35 0.0
481.3 2.3
481.86 0.0
482.37 0.0
511.31 15.5
512.03 0.0
512.5 1.2
513.04 0.0
513.6 0.0
526.34 7.3
526.89 0.0
527.44 0.0
527.96 0.0
529.17 1.1
529.72 0.0
530.27 0.0
537.24 1.9
537.79 0.0
538.35 0.0
539.01 3.2
539.55 0.0
540.11 0.0
540.63 0.0
818.25 3.4
818.79 0.0
819.35 0.0
819.87 0.0
836.2 3.6
836.77 0.0
837.28 0.0
837.83 0.0
874.53 0.7
875.1 0.0
875.62 0.0
887.4 2.0
887.95 0.0
888.49 0.0

There are 17 similar spectra:

Id Structure Score

0.77

7974

0.77

7975

0.77

0.77

5413

0.76

5691

0.76

0.75

7973

0.75

4839

0.70

0.70

5414

0.69

4799

0.67

0.67

6794

0.66

7401

0.65

7430

0.65

5692

0.56

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 570.24
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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