Structure

    redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd

Gill mucus and gill mucin O-glycosylation in healthy and amebic gill disease affected Atlantic salmon

GlyTouCan Accession Number: Loading...

Peak Intensity
347.26 9.2
348.06 1.8

Annotations:

BY b1D-Gal,p/#bcleavage--??1HexNAc,p(--??1L-Fuc,p)--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ b1D-Gal,p/#ccleavage--??1HexNAc,p(--??1L-Fuc,p)--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p/#zcleavage@360$MONO,Und,-H,0,redEnd
363.17 0.9
364.37 1.3
365.15 13.5

Annotations:

^{2,4}X_{GalNAc} redEnd--??1D-GalNAc,p/#xcleavage_2_4--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd
365.57 1.1
365.88 0.9
366.17 1.6

Annotations:

CY b1D-Gal,p/#ccleavage--??1HexNAc,p(--??1L-Fuc,p)--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p/#zcleavage@360$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p/#ycleavage@360$MONO,Und,-H,0,redEnd
371.0 2.6
387.14 2.3

Annotations:

BZ b1D-Gal,p/#bcleavage--??1HexNAc,p(--??1L-Fuc,p/#zcleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
387.34 1.9
389.26 10.8
404.21 0.9
405.23 1.7

Annotations:

BY b1D-Gal,p/#bcleavage--??1HexNAc,p(--??1L-Fuc,p/#ycleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd CZ b1D-Gal,p/#ccleavage--??1HexNAc,p(--??1L-Fuc,p/#zcleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
407.21 524.0

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd
408.45 1.2
425.33 15.8

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd
443.26 0.9
447.55 2.4
461.13 2.1
485.5 0.9
497.15 1.3
527.28 50.8
533.08 1.3
545.22 4.2
545.41 2.9
549.11 1.5

Annotations:

BZ ?1D-GalNAc,p/#bcleavage--3b1D-Gal,p--??1HexNAc,p(--??1L-Fuc,p/#zcleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
549.39 0.8
550.16 0.9
551.23 84.4

Annotations:

B b1D-Gal,p/#bcleavage--??1HexNAc,p(--??1L-Fuc,p)--??1HexNAc,p$MONO,Und,-H,0,redEnd
552.3 1.0

Annotations:

^{2,4}A_{GalNAc}Z ?1D-GalNAc,p/#acleavage_2_4--3b1D-Gal,p--??1HexNAc,p(--??1L-Fuc,p)--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
568.34 2.0
568.96 5.6
569.29 10.3

Annotations:

C b1D-Gal,p/#ccleavage--??1HexNAc,p(--??1L-Fuc,p)--??1HexNAc,p$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd
585.98 1.2
587.24 1041.8

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd
588.21 5.1
589.47 0.8
592.08 0.8
593.12 0.9
602.45 1.2
611.29 0.7
622.17 1.9
622.41 0.9
637.22 1.7
662.25 1.2
664.38 4.7
675.28 1.8
682.33 35.8
711.1 1.6
711.95 0.8
712.46 3.4

Annotations:

^{0,4}X_{GalNAc}Z redEnd--??1D-GalNAc,p/#xcleavage_0_4--3b1D-Gal,p--??1HexNAc,p(--??1L-Fuc,p/#zcleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
713.25 4.4

Annotations:

B ?1D-GalNAc,p/#bcleavage--3b1D-Gal,p--??1HexNAc,p(--??1L-Fuc,p)--??1HexNAc,p$MONO,Und,-H,0,redEnd
713.53 1.1
724.3 84.1
727.97 0.8
730.34 24.8

Annotations:

^{0,4}X_{GalNAc}Y redEnd--??1D-GalNAc,p/#xcleavage_0_4--3b1D-Gal,p--??1HexNAc,p(--??1L-Fuc,p/#ycleavage)--??1HexNAc,p$MONO,Und,-H,0,redEnd
731.15 1.4

Annotations:

C ?1D-GalNAc,p/#ccleavage--3b1D-Gal,p--??1HexNAc,p(--??1L-Fuc,p)--??1HexNAc,p$MONO,Und,-H,0,redEnd
740.39 0.9
752.79 1.3
754.2 3.9

Annotations:

ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1L-Fuc,p/#zcleavage)--??1HexNAc,p)--6b1D-GlcNAc,p/#zcleavage@360$MONO,Und,-H,0,redEnd ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1L-Fuc,p/#zcleavage)--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd
772.22 50.9

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1L-Fuc,p/#ycleavage)--??1HexNAc,p)--6b1D-GlcNAc,p/#zcleavage@360$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1L-Fuc,p/#zcleavage)--??1HexNAc,p)--6b1D-GlcNAc,p/#ycleavage@360$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1L-Fuc,p/#zcleavage)--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1L-Fuc,p/#ycleavage)--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd
777.59 0.9
790.24 40.3

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1L-Fuc,p/#ycleavage)--??1HexNAc,p)--6b1D-GlcNAc,p/#ycleavage@360$MONO,Und,-H,0,redEnd YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1L-Fuc,p/#ycleavage)--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd
791.31 7.3
791.67 1.8
819.12 0.7
828.19 2.3
828.51 0.7
858.53 0.8
867.17 1.9
867.47 2.2
885.22 0.9
885.55 3.1
888.23 0.8
916.51 1.2
918.24 8.4

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1L-Fuc,p)--??1HexNAc,p)--6b1D-GlcNAc,p/#zcleavage@360$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1L-Fuc,p)--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd
918.62 1.6
927.6 3.1
936.35 4.9

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1L-Fuc,p)--??1HexNAc,p)--6b1D-GlcNAc,p/#ycleavage@360$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1L-Fuc,p)--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd
937.19 1.4
937.57 1.6
938.3 1.2
957.33 1.8
975.36 4.6

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1L-Fuc,p/#zcleavage)--??1HexNAc,p)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd
980.25 0.7
993.42 2.5

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1L-Fuc,p/#ycleavage)--??1HexNAc,p)--6b1D-GlcNAc,p@360$MONO,Und,-H,0,redEnd
994.47 8.6
994.91 1.3
995.29 2.2
1040.06 1.0
1040.53 1.0
1049.13 2.1
1060.34 0.8
1061.15 0.8
1079.35 46.5
1089.37 1.2
1097.35 25.4
1102.09 5.0
1108.36 1.0

There are 8 similar spectra:

Id Structure Score

5749

0.99

4891

0.98

5459

0.90

5750

0.89

5458

0.89

4892

0.87

4893

0.85

4817

0.79

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1138.45
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Salmo Salar
Tissue - Gills

HPLC

Column

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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