Structure

    redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd

Gill mucus and gill mucin O-glycosylation in healthy and amebic gill disease affected Atlantic salmon

GlyTouCan Accession Number: Loading...

Peak Intensity
324.39 0.7
352.39 0.6
363.47 0.5
365.25 1.3

Annotations:

^{2,4}X_{GalNAc} redEnd--??1D-GalNAc,p/#xcleavage_2_4--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
365.74 0.7

Annotations:

C ?1HexNAc,p/#ccleavage--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
369.82 1.5
382.12 1.9

Annotations:

CY ?1D-GalNAc,p/#ccleavage--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
383.9 2.0
384.25 1.0

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
387.14 0.6

Annotations:

BZ b1D-Gal,p/#bcleavage--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
388.8 0.6
389.24 0.6
405.01 3.2

Annotations:

BY b1D-Gal,p/#bcleavage--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ b1D-Gal,p/#ccleavage--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
406.45 1.6

Annotations:

^{2,4}A_{GalNAc}Z ?1D-GalNAc,p/#acleavage_2_4--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
407.11 37.2

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
407.41 11.5
425.21 13.1

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
435.27 2.2
527.28 27.5

Annotations:

^{0,4}X_{GalNAc}Y redEnd--??1D-GalNAc,p/#xcleavage_0_4--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
544.24 1.0
545.17 1.2
549.01 1.4

Annotations:

BZ ?1D-GalNAc,p/#bcleavage--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
550.5 2.3
551.2 8.8

Annotations:

B b1D-Gal,p/#bcleavage--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
551.48 7.5
567.27 7.0

Annotations:

BY ?1D-GalNAc,p/#bcleavage--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
567.49 1.2
568.18 3.3
569.26 7.5

Annotations:

C b1D-Gal,p/#ccleavage--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
574.27 0.8
587.24 240.5

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
592.5 1.7
596.67 1.3
622.48 1.0
628.45 2.1
658.39 1.3
664.11 2.2
664.41 1.8
682.51 2.7
694.05 2.3
699.56 1.2
711.93 2.7

Annotations:

^{0,4}X_{GalNAc}Z redEnd--??1D-GalNAc,p/#xcleavage_0_4--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
712.29 0.9
713.42 0.8

Annotations:

B ?1D-GalNAc,p/#bcleavage--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
718.44 3.2
724.32 23.1
730.33 33.4

Annotations:

^{0,4}X_{GalNAc}Y redEnd--??1D-GalNAc,p/#xcleavage_0_4--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
733.5 3.8
735.32 1.3
753.11 0.7
772.28 2.4

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
772.61 2.0
773.48 2.4

Annotations:

^{2,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_2_4--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
774.23 1.1
790.12 70.1

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
790.48 43.1
792.16 4.1
792.47 2.5
811.3 2.0
827.98 2.0
837.3 2.1
847.49 3.1
867.27 9.6
867.64 1.4
885.01 3.1
885.59 0.8
890.39 1.8
915.42 2.1
916.06 1.6
916.25 1.3
917.4 2.2
918.1 1.8

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
918.37 1.7
919.35 1.3
927.31 11.7
927.58 4.9
936.28 4.4

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
938.23 1.3
956.26 0.5
975.02 6.7

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
975.44 0.6
986.39 5.0
987.78 1.1
989.25 4.9
994.4 0.9
995.43 0.7
1005.83 1.3
1031.3 1.4
1037.21 1.4
1040.61 1.2
1041.15 0.8
1045.96 0.9
1061.53 0.8
1064.2 2.0
1079.37 28.4
1091.54 4.5
1097.43 2.6
1102.32 3.1
1102.71 0.4
1131.48 3.0

There are 13 similar spectra:

Id Structure Score

5458

0.94

4817

0.93

0.93

4892

0.93

0.93

5749

0.91

5750

0.91

5455

0.90

4891

0.85

0.85

4893

0.85

0.84

5456

0.58

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1138.45
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Salmo Salar
Tissue - Gills

HPLC

Column

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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