Structure

    redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd

Gill mucus and gill mucin O-glycosylation in healthy and amebic gill disease affected Atlantic salmon

GlyTouCan Accession Number: Loading...

Peak Intensity
348.14 2.2

Annotations:

BY b1D-Gal,p/#bcleavage--4?1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd CZ b1D-Gal,p/#ccleavage--4?1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p/#zcleavage)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
366.11 1.0

Annotations:

CY b1D-Gal,p/#ccleavage--4?1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p/#ycleavage)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p/#zcleavage)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
383.99 13.9

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p/#ycleavage)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
384.31 9.1
387.3 2.4

Annotations:

BZ b1D-Gal,p/#bcleavage--4?1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
417.4 3.0
436.05 1.3

Annotations:

Z^{0,2}X_{NeuAc} redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p/#zcleavage)--6a2D-NeuAc,p/#xcleavage_0_2$MONO,Und,-H,0,redEnd
437.05 1.7
454.13 10.0

Annotations:

Y^{0,2}X_{NeuAc} redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p/#ycleavage)--6a2D-NeuAc,p/#xcleavage_0_2$MONO,Und,-H,0,redEnd
454.34 6.1
473.15 1.5
477.17 1.3
479.29 6.3
489.22 1.1
493.14 1.0
495.25 5.3

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
507.11 4.6
513.21 6.6

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
513.52 1.0
521.26 11.6
537.18 1.3
551.23 80.2

Annotations:

B b1D-Gal,p/#bcleavage--4?1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
552.24 1.3

Annotations:

^{2,4}A_{GalNAc}Z ?1D-GalNAc,p/#acleavage_2_4--3b1D-Gal,p--4?1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
555.01 1.5

Annotations:

^{0,2}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_0_2)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
568.9 1.1
569.32 4.2

Annotations:

C b1D-Gal,p/#ccleavage--4?1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
571.25 1.7
579.4 1.4
587.35 4.4
597.25 29.6
611.27 1.1

Annotations:

^{2,4}A_{Gal} b1D-Gal,p/#acleavage_2_4--4?1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
615.21 101.9

Annotations:

^{2,4}X_{Gal} redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#xcleavage_2_4)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
638.98 2.8
639.33 3.1
657.26 144.7

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p/#zcleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
658.19 2.6
675.28 455.1

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p/#ycleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
676.75 2.4
692.28 2.6
692.54 1.6
708.31 1.1
713.33 2.6

Annotations:

B ?1D-GalNAc,p/#bcleavage--3b1D-Gal,p--4?1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
720.95 1.1
734.37 3.4
752.18 2.2
752.54 2.6
782.15 2.4
789.93 1.6
790.24 2.5

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#ycleavage)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
793.88 1.1
794.46 3.8
800.44 3.3
842.3 6.4

Annotations:

Z^{0,2}X_{NeuAc} redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#zcleavage)--6a2D-NeuAc,p/#xcleavage_0_2$MONO,Und,-H,0,redEnd ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p/#zcleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
842.52 2.9
860.48 4.1

Annotations:

Y^{0,2}X_{NeuAc} redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#ycleavage)--6a2D-NeuAc,p/#xcleavage_0_2$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p/#zcleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p/#ycleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
861.37 1.9
878.21 2.0
878.48 3.8

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p(--??1HexNAc,p/#ycleavage)--??1L-Fuc,p/#ycleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
879.34 1.2
900.26 2.8
917.71 1.0
918.17 8.1

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
918.49 5.3
936.31 18.1

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
938.64 1.1
970.42 1.2
988.44 9.1
1006.37 95.0

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p(--??1HexNAc,p/#zcleavage)--??1L-Fuc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd ^{0,2}X_{NeuAc} redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p)--6a2D-NeuAc,p/#xcleavage_0_2$MONO,Und,-H,0,redEnd
1008.16 1.0
1008.33 1.4
1023.23 1.3
1027.43 1.0
1029.36 2.1
1029.61 2.0
1059.19 4.7
1063.47 9.4

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#zcleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
1064.24 4.5
1064.51 1.2
1077.38 1.2
1080.87 1.0
1081.44 28.0

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--4?1HexNAc,p(--??1HexNAc,p)--??1L-Fuc,p/#ycleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
1082.49 2.1
1083.41 9.6
1083.67 4.6
1083.92 1.8
1089.06 1.5
1089.65 1.3
1119.05 2.2
1119.41 11.5
1119.66 4.3
1123.17 1.0
1131.45 1.8
1149.39 79.8
1167.38 389.6
1185.25 9.4
1185.51 4.0
1190.35 1.8
1191.34 9.9
1191.6 4.9
1239.34 1.8

There are 7 similar spectra:

Id Structure Score

4901

0.95

5751

0.68

5463

0.63

5464

0.60

4900

0.58

5835

0.57

4899

0.56

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1226.47
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Salmo Salar
Tissue - Gills

HPLC

Column

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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