Structure

    redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd

Gill mucus and gill mucin O-glycosylation in healthy and amebic gill disease affected Atlantic salmon

GlyTouCan Accession Number: Loading...

Peak Intensity
369.49 1.6
407.08 2.2

Annotations:

YZ redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1D-Gal,p/#ycleavage)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1D-Gal,p/#zcleavage)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
425.18 2.6

Annotations:

YY redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1D-Gal,p/#ycleavage)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
463.2 1.2
477.16 2.7

Annotations:

Z^{0,2}X_{NeuAc} redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1D-Gal,p/#zcleavage)--6a2D-NeuAc,p/#xcleavage_0_2$MONO,Und,-H,0,redEnd
497.04 1.0
513.45 1.2

Annotations:

Y redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p/#ycleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
527.13 9.8

Annotations:

^{0,4}X_{GalNAc}Y redEnd--??1D-GalNAc,p/#xcleavage_0_4--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
527.43 4.3
545.3 3.2
569.04 8.5

Annotations:

YZ redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
569.28 7.6
569.54 5.8
570.67 1.0
574.34 1.5
578.28 2.5
587.27 73.2

Annotations:

YY redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
613.43 2.4
638.54 2.3
639.22 2.2

Annotations:

Z^{0,2}X_{NeuAc} redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage)--6a2D-NeuAc,p/#xcleavage_0_2$MONO,Und,-H,0,redEnd
654.35 0.9
657.24 5.6

Annotations:

Y^{0,2}X_{NeuAc} redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage)--6a2D-NeuAc,p/#xcleavage_0_2$MONO,Und,-H,0,redEnd
664.17 10.0
674.92 0.8
675.56 2.0
682.34 10.6
698.51 3.6

Annotations:

Z redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1D-Gal,p/#zcleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
712.21 1.0

Annotations:

^{0,4}X_{GalNAc}Z redEnd--??1D-GalNAc,p/#xcleavage_0_4--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
713.04 2.0

Annotations:

B ?1D-GalNAc,p/#bcleavage--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
713.24 0.8
716.23 2.4

Annotations:

Y redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1D-Gal,p/#ycleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
716.53 1.2
717.3 3.7
717.65 2.5
724.24 15.4
740.47 0.8
754.44 0.9

Annotations:

ZZ redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
766.31 1.2
772.11 3.7

Annotations:

YZ redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
772.34 1.4
790.48 5.2

Annotations:

YY redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
800.24 3.9
818.26 15.1
818.55 11.8
859.37 2.5
860.23 14.6

Annotations:

Y^{0,2}X_{NeuAc} redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6a2D-NeuAc,p/#xcleavage_0_2$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p/#zcleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
860.56 1.1
861.4 1.0
877.91 4.8
878.4 28.6

Annotations:

Y redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p/#ycleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
896.47 2.6
900.21 1.5
906.38 0.7
913.47 2.9
917.82 2.8
918.31 1.0

Annotations:

Z redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
919.37 0.9
936.35 41.5

Annotations:

Y redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
951.19 1.9
955.3 2.5
978.26 4.0
985.4 1.1
986.16 1.6
987.78 3.8
988.0 1.8
988.27 1.2
988.53 2.6
1003.55 3.6
1004.2 4.2
1006.26 36.3

Annotations:

^{0,2}X_{NeuAc} redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6a2D-NeuAc,p/#xcleavage_0_2$MONO,Und,-H,0,redEnd
1006.54 26.6
1006.77 15.8
1022.45 4.0
1029.42 2.8
1029.65 1.6
1044.71 2.9
1045.18 1.4
1045.53 1.5
1059.39 5.5
1063.41 3.1

Annotations:

Z redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
1077.43 1.9
1081.02 4.8
1081.44 8.2

Annotations:

Y redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
1082.61 9.6
1083.38 4.7
1088.87 2.5
1089.25 3.1
1107.69 2.1
1110.24 3.4
1119.48 4.0
1125.45 3.3
1141.55 1.0
1149.28 32.0
1149.69 21.5
1167.41 292.9
1185.2 4.2
1185.58 5.9
1190.2 3.5
1191.53 2.1
1228.48 3.2

There are 13 similar spectra:

Id Structure Score

5463

0.97

4900

0.92

0.92

4899

0.90

0.90

6819

0.88

5835

0.85

5753

0.63

5462

0.60

4901

0.54

0.54

5752

0.52

5751

0.50

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1226.47
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Salmo Salar
Tissue - Gills

HPLC

Column

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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