Structure

    redEnd--??1D-GalNAc,p(--??1D-GalNAc,p)--6a2D-NeuGc,p$MONO,Und,-H,0,redEnd

Rainbow trout mucus O-glycosylation

GlyTouCan Accession Number: Loading...

Peak Intensity
203.91 2.1

Annotations:

ZY redEnd--??1D-GalNAc,p(--??1D-GalNAc,p/#zcleavage)--6a2D-NeuGc,p/#ycleavage$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--??1D-GalNAc,p/#ycleavage)--6a2D-NeuGc,p/#zcleavage$MONO,Und,-H,0,redEnd
217.94 1.1
222.1 1.7

Annotations:

YY redEnd--??1D-GalNAc,p(--??1D-GalNAc,p/#ycleavage)--6a2D-NeuGc,p/#ycleavage$MONO,Und,-H,0,redEnd
230.23 1.5
235.91 3.3
244.12 1.0
255.57 1.5
256.16 1.3
287.76 1.0
288.2 1.9
292.05 3.1
298.94 1.2
303.99 1.9
306.13 237.9

Annotations:

B ?1D-GalNAc,p/#bcleavage--6a2D-NeuGc,p$MONO,Und,-H,0,redEnd
307.1 19.3
312.12 1.6
316.52 1.4
321.64 1.1
323.75 1.4

Annotations:

C ?1D-GalNAc,p/#ccleavage--6a2D-NeuGc,p$MONO,Und,-H,0,redEnd
324.1 5.6

Annotations:

C ?1D-GalNAc,p/#ccleavage--6a2D-NeuGc,p$MONO,Und,-H,0,redEnd
329.31 2.6
342.19 1.9
346.14 4.2
346.42 1.0
348.09 8.1
348.32 3.1
348.72 1.7
350.09 1.4
366.26 1.0
371.39 1.3
373.02 2.4
384.2 237.5
385.12 1.8
405.17 2.2
405.42 1.6
407.52 7.7

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-GalNAc,p)--6a2D-NeuGc,p/#zcleavage$MONO,Und,-H,0,redEnd
408.2 2.2
417.36 2.8
425.31 25.9

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-GalNAc,p)--6a2D-NeuGc,p/#ycleavage$MONO,Und,-H,0,redEnd
426.17 3.8

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-GalNAc,p)--6a2D-NeuGc,p/#ycleavage$MONO,Und,-H,0,redEnd
426.55 1.3
435.28 1.3
441.36 1.0
445.32 1.8
446.31 1.3
461.06 4.9
461.39 2.8
467.09 4.4
467.36 1.8
467.62 1.0
468.44 2.0
475.24 1.7
479.34 11.3
479.59 4.1
485.37 1.8
493.21 8.4
494.27 2.2
494.85 1.5
495.29 6.1
495.75 1.1
496.34 1.2
508.07 1.5
510.29 9.1

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-GalNAc,p/#zcleavage)--6a2D-NeuGc,p$MONO,Und,-H,0,redEnd
512.13 1.5

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-GalNAc,p/#zcleavage)--6a2D-NeuGc,p$MONO,Und,-H,0,redEnd
513.39 5.5
514.24 1.3
518.47 2.9
520.55 1.0
521.21 48.5
521.51 20.3
529.15 4.0

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-GalNAc,p/#ycleavage)--6a2D-NeuGc,p$MONO,Und,-H,0,redEnd
529.43 2.5

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-GalNAc,p/#ycleavage)--6a2D-NeuGc,p$MONO,Und,-H,0,redEnd
530.49 8.7
531.37 1.6
549.05 0.9
550.61 1.3
551.05 2.2
569.3 58.9
587.25 35.4
588.4 14.1
608.43 1.4
611.05 1.3
624.03 1.7
625.35 2.5
628.95 1.0
638.32 120.5
639.47 21.3
654.31 1.6
655.11 1.4
656.31 5.0
657.36 1.1
669.17 1.8
670.04 1.5
671.51 2.6
671.76 1.4
688.48 1.2
696.45 1.5
701.93 4.0
774.63 1.8
854.37 1.5

There are 13 similar spectra:

Id Structure Score

7403

0.97

7432

0.97

6715

0.72

5707

0.72

4846

0.72

0.72

6801

0.70

7989

0.70

5425

0.70

6714

0.64

5706

0.64

8114

0.61

3751

0.58

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 731.28
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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