Structure

    redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd

Rainbow trout mucus O-glycosylation

GlyTouCan Accession Number: Loading...

Peak Intensity
266.18 0.3
281.34 0.2
293.38 0.2
298.7 0.3
299.32 0.3
304.24 0.5
305.86 0.5
310.44 0.4
324.13 2.8
329.17 1.6

Annotations:

BZ ?1D-GalNAc,p/#bcleavage--??1D-GlcNAc,p(--??1D-Gal,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
330.27 0.3

Annotations:

BZ ?1D-GalNAc,p/#bcleavage--??1D-GlcNAc,p(--??1D-Gal,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
341.07 1.1
342.19 0.5
343.08 0.3
348.04 0.2

Annotations:

BY ?1D-GalNAc,p/#bcleavage--??1D-GlcNAc,p(--??1D-Gal,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--??1D-GlcNAc,p(--??1D-Gal,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
352.77 0.2
353.47 0.2
359.22 0.3
364.0 0.3

Annotations:

BY ?1D-GalNAc,p/#bcleavage--??1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--??1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
366.34 1.6

Annotations:

CY ?1D-GalNAc,p/#ccleavage--??1D-GlcNAc,p(--??1D-Gal,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
371.22 1.7
372.06 0.5
372.61 0.2
384.27 11.3

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
385.27 0.8
389.01 0.3
389.4 0.6
390.31 0.4
391.15 0.3
401.5 0.3
419.41 0.4
437.41 0.5
443.24 0.2
461.3 1.2
462.4 0.3
475.52 0.2
478.77 0.3
479.22 1.2
479.63 0.5
491.49 0.6
492.25 0.7
492.6 0.5
503.39 0.6
509.27 4.1

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
510.38 2.3

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
511.33 0.6
519.7 0.2
521.22 3.8
522.34 0.2
526.41 0.3
528.86 0.2

Annotations:

C ?1D-GalNAc,p/#ccleavage--??1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
533.28 1.5
534.2 0.2
534.44 0.3

Annotations:

ZZ redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??1D-GlcNAc,p(--??1D-Gal,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
551.26 10.1

Annotations:

ZZ redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p(--??1D-Gal,p/#zcleavage)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd ZZ redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
552.36 1.3

Annotations:

YZ redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p(--??1D-Gal,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??1D-GlcNAc,p(--??1D-Gal,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
553.34 1.9

Annotations:

YZ redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p(--??1D-Gal,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??1D-GlcNAc,p(--??1D-Gal,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
553.74 0.2

Annotations:

YZ redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p(--??1D-Gal,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??1D-GlcNAc,p(--??1D-Gal,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
569.15 0.5

Annotations:

YZ redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p(--??1D-Gal,p/#ycleavage)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p(--??1D-Gal,p/#zcleavage)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
569.58 0.2

Annotations:

YZ redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p(--??1D-Gal,p/#ycleavage)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p(--??1D-Gal,p/#zcleavage)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
570.51 0.4

Annotations:

YY redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p(--??1D-Gal,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
571.2 1.3

Annotations:

YY redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p(--??1D-Gal,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
588.26 0.2
614.53 0.3
637.08 0.2
655.18 0.2
673.59 1.2
675.8 0.2
685.36 0.2
689.2 0.2
690.51 0.1
691.73 0.2
713.75 0.2
714.29 0.2

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p(--??1D-Gal,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
714.82 0.7

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p(--??1D-Gal,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
715.32 7.3

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p(--??1D-Gal,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
715.57 3.2

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p(--??1D-Gal,p/#zcleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
716.47 1.5
731.15 0.7

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
731.48 0.4

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
732.29 0.4

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p(--??1D-Gal,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
732.52 0.3

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p(--??1D-Gal,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
733.12 5.2

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p(--??1D-Gal,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
733.47 14.8

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p(--??1D-Gal,p/#ycleavage)--??1L-Fuc,p$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
734.33 5.7
735.58 0.5
749.3 0.9

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
750.36 0.9
750.57 0.5
755.38 0.2
774.18 0.4
774.45 0.9
775.99 0.2
802.94 0.2
835.54 4.8
836.41 4.4
846.97 0.1
853.55 2.6
854.36 1.2
854.64 1.2

There are 19 similar spectra:

Id Structure Score

5573

0.93

5574

0.90

6737

0.89

5787

0.87

0.86

6591

0.86

3881

0.76

0.71

7652

0.71

7668

0.71

3975

0.62

3125

0.58

4265

0.58

5250

0.58

7223

0.54

0.54

0.53

4863

0.53

5438

0.51

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 894.35
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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