Structure

    redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd

Rainbow trout mucus O-glycosylation

GlyTouCan Accession Number: Loading...

Peak Intensity
240.81 5.3

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1D-Gal,p--??1S$MONO,Und,-H,0,redEnd
241.08 2.0

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1D-Gal,p--??1S$MONO,Und,-H,0,redEnd
241.87 0.4

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1D-Gal,p--??1S$MONO,Und,-H,0,redEnd
257.07 0.3
258.74 0.3

Annotations:

C ?1D-GalNAc,p/#ccleavage--??1D-Gal,p--??1S$MONO,Und,-H,0,redEnd
264.2 0.4
267.07 0.4
326.16 0.7
344.92 0.4
356.26 0.4
368.03 0.7
368.28 0.3
369.29 0.3
370.15 15.9
371.2 1.3
383.16 0.3

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S/#lcleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
384.28 1.3

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S/#lcleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
384.89 0.4

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S/#lcleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
388.17 0.5
404.21 0.7
405.28 0.2
417.27 1.2
422.38 0.3
430.56 0.3
434.3 0.4
444.2 1.1
445.17 0.9

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
445.56 0.9

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
446.26 3.2

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
462.36 6.9
463.21 28.4

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
464.23 3426.7

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
465.24 410.5
466.36 3.9
467.17 1.1
478.11 0.3
489.96 0.3
490.47 1.1
493.42 0.7
511.33 1.4
513.32 1.0

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
513.57 0.3

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
514.51 0.6
517.07 0.4
519.51 0.5
525.2 0.6
534.33 1.5
548.33 0.3
549.24 0.3
554.18 0.6
558.04 1.2
560.87 0.2
566.01 0.3
571.01 0.7
572.22 0.3
576.76 0.8
583.09 0.5
585.01 0.2
592.83 0.4
593.15 1.8
597.18 1.6
598.37 0.3
607.45 0.3
610.32 0.4
614.26 0.5
615.23 0.5
616.11 1.5
635.25 0.3
639.34 0.7
654.53 0.6
655.95 0.2
656.22 0.7
657.27 9.6
657.62 4.9
658.13 0.5
658.45 3.1
663.96 0.4
667.25 0.2
669.28 0.6
672.34 0.8
673.2 0.3
673.72 0.5
674.46 4.6

Annotations:

redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S/#lcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
675.37 200.0

Annotations:

redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S/#lcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
676.36 51.0
678.56 0.2
680.22 0.4
682.4 0.5
692.17 0.2
693.46 0.4
694.2 0.8
695.41 4.5
696.05 1.2
696.37 1.7
710.46 5.9
711.39 210.4
712.4 55.8
721.88 0.4
781.32 0.4
856.97 0.4

There are 19 similar spectra:

Id Structure Score

7695

1.00

1.00

7170

1.00

7683

1.00

7690

1.00

1.00

7405

1.00

7434

1.00

5429

0.99

5713

0.99

3497

0.99

4850

0.99

0.99

6586

0.99

0.99

7626

0.99

5714

0.99

6587

0.99

0.99

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 754.21
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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