Structure

    redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd

Gill mucus and gill mucin O-glycosylation in healthy and amebic gill disease affected Atlantic salmon

GlyTouCan Accession Number: Loading...

Peak Intensity
347.36 3.0
348.22 12.7

Annotations:

B b1D-Gal,p/#bcleavage--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
365.21 4.3

Annotations:

^{2,4}X_{GalNAc} redEnd--??1D-GalNAc,p/#xcleavage_2_4--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
365.6 2.0
365.92 2.2
366.28 4.8

Annotations:

C b1D-Gal,p/#ccleavage--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
371.28 3.6
383.16 2.1
384.19 53.8

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
387.28 6.4
389.18 4.0
407.17 413.2

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
408.09 3.5
408.34 1.8
421.38 1.8
425.22 53.4

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
426.35 1.7
449.13 1.3
461.34 3.1
465.97 1.4
469.05 1.8
479.12 8.1
487.25 1.4
495.06 1.5
503.14 2.5
504.22 1.7
509.03 1.8

Annotations:

^{0,4}X_{GalNAc}Z redEnd--??1D-GalNAc,p/#xcleavage_0_4--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
509.28 1.6
509.56 1.3
510.1 1.4

Annotations:

B ?1D-GalNAc,p/#bcleavage--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
521.23 13.3
526.32 2.1
527.25 87.2

Annotations:

^{0,4}X_{GalNAc}Y redEnd--??1D-GalNAc,p/#xcleavage_0_4--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
528.21 3.6

Annotations:

C ?1D-GalNAc,p/#ccleavage--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
533.17 1.7
545.15 8.4
545.48 5.2
550.13 1.8
550.36 2.4
551.23 277.8

Annotations:

ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
552.52 1.4
554.28 1.7
557.42 2.2
560.25 1.9
569.24 29.1

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
571.5 1.4
573.09 2.0
582.02 1.4
585.36 1.5
587.21 795.0

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
588.21 17.4
590.28 2.2
597.38 1.3
622.57 2.5
642.45 1.3
643.89 1.7
644.39 2.3
664.3 30.7
665.13 2.6
670.21 1.3
675.17 5.6
676.47 1.6
682.25 66.6
689.7 1.9
695.38 2.7
705.99 1.4
713.11 2.6
724.3 176.4
730.05 2.1
731.88 1.9
733.32 4.4

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
736.2 1.6
754.27 6.5
772.32 68.1

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
772.85 1.6
773.18 1.3
785.26 6.4
790.2 2.7

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
791.38 16.3
792.03 3.2
802.27 2.0
808.21 1.3
816.37 2.1
836.02 2.5
838.34 1.8
858.08 1.6
858.41 1.7
865.35 2.6
873.36 3.4
874.3 4.8
876.36 29.5
881.14 1.6
891.77 2.5
892.15 1.5
894.21 4.7
894.41 3.1
899.29 17.4
900.13 5.2
900.57 1.7
936.39 4.2

There are 17 similar spectra:

Id Structure Score

5442

0.97

6812

0.95

7174

0.95

5730

0.95

4810

0.94

0.94

5440

0.91

4867

0.88

0.88

5727

0.88

7173

0.87

5728

0.86

6811

0.86

4809

0.85

0.85

7150

0.85

5732

0.84

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 935.38
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Salmo Salar
Tissue - Gills

HPLC

Column

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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