Structure

    redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1D-GalNAc,p--3a1L-Fuc,p$MONO,Und,-H,0,redEnd

Rainbow trout gastrointestinal mucus, mucin production, mucin glycosylation and response to lipopolysaccharide

GlyTouCan Accession Number: Loading...

Peak Intensity
281.27 2.3
291.21 3.5
299.11 3.8
299.45 2.8
305.26 2.1
329.18 4.5
341.17 3.1
347.21 10.2
348.34 2.5

Annotations:

B ?1D-Gal,p/#bcleavage--??1D-GalNAc,p--3a1L-Fuc,p$MONO,Und,-H,0,redEnd
359.16 2.2
364.15 12.7

Annotations:

BY ?1D-GalNAc,p/#bcleavage--3a1D-GalNAc,p--??1D-Gal,p--??1D-GalNAc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--3a1D-GalNAc,p--??1D-Gal,p--??1D-GalNAc,p/#zcleavage$MONO,Und,-H,0,redEnd BY a1D-GalNAc,p/#bcleavage--??1D-Gal,p--??1D-GalNAc,p--3a1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ a1D-GalNAc,p/#ccleavage--??1D-Gal,p--??1D-GalNAc,p--3a1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
365.33 13.2
384.28 12.2
387.31 2.0
388.11 3.6
407.18 22.5

Annotations:

Z redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
407.5 4.7
414.24 1.8
425.09 8.5

Annotations:

Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
425.38 7.4
426.68 2.8
431.26 3.0
437.27 3.5
442.11 2.8
459.22 3.9
461.47 1.7
465.04 3.7
467.17 2.6
469.16 3.3
478.99 3.2
483.41 3.2
485.09 5.1
485.32 2.0
501.23 2.4
521.45 3.3
523.23 4.9
527.3 172.8
528.19 12.9

Annotations:

C a1D-GalNAc,p/#ccleavage--??1D-Gal,p--??1D-GalNAc,p--3a1L-Fuc,p$MONO,Und,-H,0,redEnd
535.28 2.2
545.41 4.1
550.37 3.3
551.06 6.1
551.41 27.2
559.81 3.1
569.26 13.4

Annotations:

Z redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1D-GalNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
569.5 7.0
569.8 3.0
571.12 3.1
574.49 2.0
587.31 809.8

Annotations:

Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1D-GalNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
588.26 26.7
588.63 6.3
598.51 2.7
622.36 13.2
622.64 2.0
623.18 2.3
653.67 3.4
664.42 66.0
665.56 6.0
675.22 2.7
682.51 40.9
682.73 10.2
688.56 2.1
704.08 1.7
708.21 2.9
712.48 10.7
713.4 8.1

Annotations:

B ?1D-GalNAc,p/#bcleavage--3a1D-GalNAc,p--??1D-Gal,p--??1D-GalNAc,p--3a1L-Fuc,p$MONO,Und,-H,0,redEnd
714.45 6.0
723.42 5.0
724.42 166.2
725.21 4.2
725.52 4.4
754.34 8.2
754.68 6.3
765.31 3.9
772.43 19.9

Annotations:

Z redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1D-GalNAc,p--3a1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
773.31 5.5

Annotations:

^{2,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_2_4--3a1D-GalNAc,p--??1D-Gal,p--??1D-GalNAc,p--3a1L-Fuc,p$MONO,Und,-H,0,redEnd
774.49 2.4
783.51 2.2
790.42 2.9

Annotations:

Y redEnd--??1D-GalNAc,p--3a1D-GalNAc,p--??1D-Gal,p--??1D-GalNAc,p--3a1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
791.23 3.2
791.76 2.7
801.39 5.9
812.43 2.0
829.18 3.4
836.23 5.7
845.49 3.4
858.24 2.0
873.26 5.1
876.55 30.8
877.35 3.1
877.62 1.9
888.94 3.6
893.38 2.4
894.27 7.9
894.51 4.9
895.29 4.1
900.21 2.6
905.41 2.8

There are 14 similar spectra:

Id Structure Score

7150

0.99

4867

0.98

5727

0.98

4809

0.97

5728

0.96

7174

0.95

5732

0.95

5440

0.94

7173

0.86

6812

0.86

5441

0.86

4810

0.86

5730

0.82

5442

0.81

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 935.38
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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