Structure

    redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd

Gill mucus and gill mucin O-glycosylation in healthy and amebic gill disease affected Atlantic salmon

GlyTouCan Accession Number: Loading...

Peak Intensity
262.26 1.3

Annotations:

^{0,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_4--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
275.99 1.6
309.15 2.4
312.14 1.8
328.93 2.2
329.27 3.9
344.97 1.3
347.27 6.2
348.06 3.0

Annotations:

B b1D-Gal,p/#bcleavage--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
348.25 2.9
352.34 2.0
359.2 4.7
363.96 2.9

Annotations:

BY ?1D-GalNAc,p/#bcleavage--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
365.15 12.7

Annotations:

^{2,4}X_{GalNAc} redEnd--??1D-GalNAc,p/#xcleavage_2_4--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
365.42 6.2
366.34 10.3

Annotations:

C b1D-Gal,p/#ccleavage--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
371.2 4.3
379.17 1.3
383.97 6.0

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
384.19 5.5
384.42 2.1
387.15 6.4
389.25 8.1
407.19 527.6

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
408.19 5.3
409.33 2.3
425.21 65.7

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
434.08 1.4
434.97 2.6
443.46 3.1
461.4 3.1
479.33 3.2
499.45 1.4
509.28 2.5

Annotations:

^{0,4}X_{GalNAc}Z redEnd--??1D-GalNAc,p/#xcleavage_0_4--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
510.22 1.3

Annotations:

B ?1D-GalNAc,p/#bcleavage--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
519.14 1.9
521.31 6.3
522.37 1.4
527.23 55.7

Annotations:

^{0,4}X_{GalNAc}Y redEnd--??1D-GalNAc,p/#xcleavage_0_4--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
528.14 2.2

Annotations:

C ?1D-GalNAc,p/#ccleavage--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
545.29 19.5
546.55 1.7
550.47 1.6
551.22 104.2

Annotations:

ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
553.5 1.3
556.16 1.3
569.26 17.1

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
570.32 1.4

Annotations:

^{2,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_2_4--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
574.13 2.9
587.23 742.9

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
588.24 17.2
590.29 1.6
604.36 2.2
622.16 4.9
622.53 3.9
624.12 2.0
624.6 1.4
625.4 5.8
642.22 1.4
664.38 25.9
665.34 1.7
670.29 1.5
682.27 103.3
695.64 1.3
701.45 1.8
706.39 2.0
712.26 5.7
713.38 1.8
715.0 1.9
715.27 6.0

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
724.27 163.7
728.28 1.9
730.13 4.1
733.06 2.8
733.35 7.1

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
734.01 2.3
752.48 1.9
754.17 2.7
762.29 1.5
767.22 1.9
772.27 53.7

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
785.17 1.7
788.34 1.9
791.39 5.0
816.39 2.0
848.26 1.7
858.12 2.6
862.54 1.4
876.31 58.8
877.23 1.5
878.71 1.8
882.79 1.6
891.08 1.9
894.42 11.9
895.56 1.3
898.97 5.6
899.19 6.3
899.46 3.4
900.35 9.6
941.49 1.6

There are 15 similar spectra:

Id Structure Score

5730

0.98

4810

0.98

6812

0.98

5441

0.97

7174

0.93

5728

0.83

4867

0.82

5727

0.82

5440

0.82

6811

0.81

4809

0.81

5732

0.81

7150

0.81

7173

0.76

5581

0.55

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 935.38
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Salmo Salar
Tissue - Gills

HPLC

Column

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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