Structure

    redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1HexNAc,p$MONO,Und,-H,0,redEnd

Rainbow trout mucus O-glycosylation

GlyTouCan Accession Number: Loading...

Peak Intensity
299.09 4.1
312.16 0.8
316.99 3.5
317.42 1.0
329.25 0.9
347.14 4.4

Annotations:

B b1D-Gal,p/#bcleavage--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
348.46 0.8

Annotations:

B b1D-Gal,p/#bcleavage--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
364.19 10.8

Annotations:

BY ?1D-GalNAc,p/#bcleavage--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd CZ ?1D-GalNAc,p/#ccleavage--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
365.26 6.7

Annotations:

C b1D-Gal,p/#ccleavage--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
366.13 1.2

Annotations:

C b1D-Gal,p/#ccleavage--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
384.18 1.3

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
387.3 0.8
389.07 1.8
389.41 1.0
402.46 0.9
406.96 3.2

Annotations:

^{2,4}A_{GalNAc}Z ?1D-GalNAc,p/#acleavage_2_4--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1HexNAc,p$MONO,Und,-H,0,redEnd
407.31 11.9

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1HexNAc,p$MONO,Und,-H,0,redEnd
408.13 3.1

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1HexNAc,p$MONO,Und,-H,0,redEnd
425.26 14.7

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1HexNAc,p$MONO,Und,-H,0,redEnd
426.33 2.6
437.06 0.9
460.1 0.9
467.32 2.0

Annotations:

^{2,5}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_2_5(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1HexNAc,p$MONO,Und,-H,0,redEnd
479.2 3.7
509.89 0.9

Annotations:

B ?1D-GalNAc,p/#bcleavage--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
521.13 0.9

Annotations:

^{1,5}A_{GalNAc}Z ?1D-GalNAc,p/#acleavage_1_5(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1HexNAc,p$MONO,Und,-H,0,redEnd
524.99 1.0
527.29 132.8

Annotations:

C ?1D-GalNAc,p/#ccleavage--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
528.28 19.4

Annotations:

C ?1D-GalNAc,p/#ccleavage--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
528.67 2.5

Annotations:

C ?1D-GalNAc,p/#ccleavage--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
544.97 4.6
545.28 1.7
545.52 1.2
546.35 1.1
549.27 5.9
549.59 1.5
550.3 6.9

Annotations:

ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6b1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
551.21 3.6

Annotations:

ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6b1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
551.5 4.5

Annotations:

ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6b1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
552.32 0.8
567.18 0.8
569.23 13.5

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6b1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6b1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1HexNAc,p$MONO,Und,-H,0,redEnd ^{2,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_2_4--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
569.6 4.3

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6b1HexNAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6b1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6b1HexNAc,p$MONO,Und,-H,0,redEnd ^{2,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_2_4--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
570.48 4.4

Annotations:

^{2,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_2_4--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
587.31 759.2

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6b1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6b1HexNAc,p$MONO,Und,-H,0,redEnd
588.31 124.1
589.96 0.8
605.31 1.0
611.41 0.8

Annotations:

^{1,3}A_{GalNAc} ?1D-GalNAc,p/#acleavage_1_3--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
617.47 1.3
621.98 1.5
622.34 3.6
623.52 2.2
624.49 1.0
627.48 0.8
646.35 0.8
647.56 0.9
653.65 0.9

Annotations:

^{2,5}A_{GalNAc}Z ?1D-GalNAc,p/#acleavage_2_5(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6b1HexNAc,p$MONO,Und,-H,0,redEnd
664.38 48.9
665.39 15.5
675.91 1.2
676.42 2.5
682.26 35.3
682.69 17.7
683.41 9.8
695.31 1.5
706.32 1.3
712.41 9.5
712.74 1.0
713.45 6.2
714.26 3.3
724.38 162.9

Annotations:

^{1,5}A_{GalNAc}Z ?1D-GalNAc,p/#acleavage_1_5(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6b1HexNAc,p$MONO,Und,-H,0,redEnd
725.44 30.9
737.36 1.3
752.44 1.1
754.54 4.0
755.34 3.2
772.54 27.9

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6b1HexNAc,p$MONO,Und,-H,0,redEnd
773.44 4.9
773.76 1.1
786.32 0.9
790.14 1.8

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6b1HexNAc,p$MONO,Und,-H,0,redEnd
790.54 1.0

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6b1HexNAc,p$MONO,Und,-H,0,redEnd
791.41 28.7
792.28 7.9
816.22 2.0
828.5 0.8
834.42 0.8
856.52 0.8
858.13 1.4
858.71 0.9
876.0 7.7
876.35 19.0
876.61 12.7
877.54 9.5
894.13 2.3
894.44 4.4
894.66 2.7
895.19 2.2
900.57 1.1

There are 14 similar spectra:

Id Structure Score

4867

0.99

5727

0.99

6811

0.99

4809

0.99

5728

0.96

5732

0.96

7174

0.95

5440

0.93

6812

0.86

7173

0.86

4810

0.85

5441

0.85

5730

0.83

5442

0.81

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 935.38
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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