Structure

    redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--4b1D-Gal,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd

The O-glycome of human nigrostriatal tissue and its alteration in Parkinsons disease

GlyTouCan Accession Number: Loading...

Peak Intensity
290.17 6.9

Annotations:

B b1D-Gal,p/#bcleavage--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
347.14 1.2
366.24 0.8

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
388.96 2.7

Annotations:

ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--4b1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
389.29 5.8

Annotations:

ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--4b1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
390.21 0.8
407.35 5.7

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--4b1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--4b1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
408.22 1.4
424.33 1.0

Annotations:

^{0,4}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_0_4--6b1D-GlcNAc,p--4b1D-Gal,p--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
509.29 1.1
527.2 3.6
528.18 1.1
551.03 0.7

Annotations:

ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--4b1D-Gal,p--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
551.35 0.7

Annotations:

ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--4b1D-Gal,p--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
569.3 51.9

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--4b1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--4b1D-Gal,p--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--4b1D-Gal,p--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
570.21 6.0
570.5 1.7
587.32 22.4

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--4b1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--4b1D-Gal,p--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
588.32 5.1
627.27 1.1

Annotations:

^{1,5}A_{GlcNAc} b1D-GlcNAc,p/#acleavage_1_5--4b1D-Gal,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
653.4 1.0
654.32 1.2
655.43 0.9

Annotations:

B ?1D-GalNAc,p/#bcleavage--6b1D-GlcNAc,p--4b1D-Gal,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
676.44 0.8
689.34 4.1
699.21 0.7
701.85 0.7
707.46 0.7
727.27 0.8
731.36 4.0

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--4b1D-Gal,p--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
732.31 1.2
745.48 3.4

Annotations:

^{0,3}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_3--6b1D-GlcNAc,p--4b1D-Gal,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
749.36 67.1

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--4b1D-Gal,p--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
750.36 13.6
756.37 1.0
758.35 0.7
782.81 0.9
783.4 1.7
783.75 1.0
784.33 0.7
785.6 0.8
791.21 1.2
791.46 1.3
800.47 0.9
801.08 0.7
801.49 2.1
816.41 1.6
819.57 1.2
835.13 1.3
835.48 1.0
842.47 5.5
843.47 1.6
857.54 2.2
857.85 0.7
859.26 0.7
860.38 1.6

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6b1D-GlcNAc,p--4b1D-Gal,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
861.27 0.9
866.89 1.4
878.34 1.9

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6b1D-GlcNAc,p--4b1D-Gal,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
879.22 1.9
879.52 3.7
880.45 1.1
885.24 0.6
887.43 4.6
887.88 1.8
914.35 0.8
932.45 0.7
937.06 0.9

Annotations:

^{0,2}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_2(--3b1D-Gal,p)--6b1D-GlcNAc,p--4b1D-Gal,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
938.47 0.8
939.74 1.5
939.99 0.7
940.48 1.1
941.54 1.2
957.55 0.8
958.52 0.7
959.21 1.1
962.42 54.0
963.43 19.2
967.49 0.6
976.46 0.8
978.57 0.7
979.37 0.9
979.7 0.9
980.32 17.0
980.62 12.4
981.7 3.8
986.44 0.8
990.35 7.8
990.68 1.8
990.97 2.1
991.85 0.7
993.58 0.7
996.42 2.3
997.53 1.1
1003.39 1.0
1003.58 0.7
1004.47 2.6
1005.3 1.7
1008.79 0.8
1009.63 1.1

There are 39 similar spectra:

Id Structure Score

3511

0.98

5449

0.96

4249

0.96

5241

0.96

3138

0.96

5804

0.94

4248

0.92

5240

0.92

6593

0.92

4212

0.92

3980

0.92

3894

0.91

4085

0.91

5590

0.91

3510

0.91

5191

0.91

5179

0.90

7413

0.90

7442

0.90

5739

0.89

5210

0.88

4877

0.88

5185

0.88

5448

0.86

3981

0.85

5803

0.80

7229

0.79

7228

0.78

3766

0.77

5740

0.77

5184

0.74

4250

0.73

5242

0.73

5741

0.70

5805

0.69

5186

0.67

7632

0.63

3979

0.59

4084

0.58

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1039.39
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Homo Sapiens
Tissue - Brain

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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