Structure

    redEnd--??1D-GalNAc,p(--??1D-Gal,p--??2D-NeuAc,p)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd

Rainbow trout mucus O-glycosylation

GlyTouCan Accession Number: Loading...

Peak Intensity
290.21 6.4

Annotations:

B ?1D-Gal,p/#bcleavage--??2D-NeuAc,p$MONO,Und,-H,0,redEnd B ?1D-GalNAc,p/#bcleavage--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
290.72 0.4

Annotations:

B ?1D-Gal,p/#bcleavage--??2D-NeuAc,p$MONO,Und,-H,0,redEnd B ?1D-GalNAc,p/#bcleavage--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
308.09 0.3

Annotations:

C ?1D-Gal,p/#ccleavage--??2D-NeuAc,p$MONO,Und,-H,0,redEnd C ?1D-GalNAc,p/#ccleavage--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
342.12 0.6
344.08 0.3
366.56 0.4

Annotations:

YZ redEnd--??1D-GalNAc,p(--??1D-Gal,p--??2D-NeuAc,p/#ycleavage)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1D-Gal,p--??2D-NeuAc,p/#zcleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
380.37 0.5
384.22 0.6

Annotations:

YY redEnd--??1D-GalNAc,p(--??1D-Gal,p--??2D-NeuAc,p/#ycleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
386.04 0.4
405.17 0.3
425.15 0.5
426.08 0.3
454.16 0.3
469.42 0.6

Annotations:

C ?1D-GalNAc,p/#ccleavage--??1D-Gal,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
470.98 0.4

Annotations:

C ?1D-GalNAc,p/#ccleavage--??1D-Gal,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
477.03 0.5
477.49 0.4
491.19 0.3
495.0 0.3

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
507.55 0.3
511.13 0.3
513.01 0.3

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
514.47 0.9
537.35 1.5
539.41 1.9
539.89 0.5
540.7 0.4
541.29 0.5
555.27 0.3
563.26 0.4
567.37 0.7
581.39 9.7
582.07 1.8
582.44 0.5
583.08 0.5
588.11 0.4
597.13 1.9
597.43 3.1
601.68 0.3
614.24 0.5
615.49 0.3
631.51 0.4
633.06 0.5
640.15 0.3
641.17 0.7
643.46 0.3
644.35 0.3
655.23 1.3
656.08 0.5
656.48 2.7

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p--??2D-NeuAc,p)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--??1D-Gal,p--??2D-NeuAc,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
657.32 32.3

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p--??2D-NeuAc,p)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--??1D-Gal,p--??2D-NeuAc,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
658.06 0.3

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p--??2D-NeuAc,p)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--??1D-Gal,p--??2D-NeuAc,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
672.52 0.4
673.14 6.0
673.64 5.9
674.34 26.4

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??2D-NeuAc,p)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??2D-NeuAc,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
675.33 1523.9

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??2D-NeuAc,p)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??2D-NeuAc,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
676.4 45.8
677.1 1.6
677.45 2.4
678.39 0.3
698.21 1.1
700.51 0.7
704.01 0.7
716.36 21.7
717.28 2.9
717.65 2.0
718.45 1.1
748.76 0.5
770.53 0.3
777.56 0.3
790.82 0.6
791.5 0.5
804.91 0.6
828.5 0.6
831.67 0.4
833.24 0.9
875.33 0.3
877.49 0.5
878.53 0.3
888.66 0.8
894.37 0.3
903.85 0.4
904.43 1.4
904.66 0.3
906.07 0.9
906.53 2.0
921.34 0.7
922.41 25.9
923.63 1.1
926.42 0.7
928.99 0.3
929.53 0.3
930.25 2.4
930.62 4.0
930.92 1.1
933.9 0.4
936.22 0.5
936.58 0.5
939.12 0.9

There are 31 similar spectra:

Id Structure Score

7184

1.00

4208

1.00

6592

1.00

7175

1.00

5586

1.00

6814

1.00

7629

1.00

5443

1.00

4083

1.00

7394

1.00

7423

1.00

5735

1.00

5521

1.00

4245

1.00

5237

1.00

3505

1.00

5796

1.00

4812

1.00

7238

1.00

5177

1.00

5207

1.00

3763

1.00

5183

1.00

7684

0.98

7691

0.98

7696

0.98

6813

0.97

3977

0.97

5189

0.97

7628

0.96

7153

0.58

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 965.35
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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