Structure

    redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd

Rainbow trout mucus O-glycosylation

GlyTouCan Accession Number: Loading...

Peak Intensity
290.12 24.5

Annotations:

B ?1D-GalNAc,p/#bcleavage--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
291.13 1.2

Annotations:

^{1,4}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_1_4--3b1D-Gal,p--??1HexNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
308.05 3.0

Annotations:

C ?1D-GalNAc,p/#ccleavage--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
348.25 1.3

Annotations:

B b1D-Gal,p/#bcleavage--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd ZZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
366.1 3.7

Annotations:

C b1D-Gal,p/#ccleavage--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
384.23 14.2

Annotations:

YY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
385.34 1.8
385.7 0.5
391.29 0.6
417.35 1.6
435.31 1.0
436.14 1.0
453.04 0.9
454.17 1.5
454.43 0.6
461.43 0.7
477.26 3.9

Annotations:

^{1,4}A_{GalNAc}Z ?1D-GalNAc,p/#acleavage_1_4--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage$MONO,Und,-H,0,redEnd
478.44 0.6
479.21 5.8
479.48 1.6
480.29 0.5
493.3 0.7
495.24 4.3

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#zcleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd ^{1,4}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_1_4--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage$MONO,Und,-H,0,redEnd
496.27 0.8
503.21 0.6
512.88 1.0

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
513.49 1.4

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p/#ycleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
514.23 1.7
514.54 0.7
521.3 4.0
522.06 0.7
553.45 1.0

Annotations:

^{0,2}A_{GalNAc}Z ?1D-GalNAc,p/#acleavage_0_2(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
555.56 1.0

Annotations:

^{2,5}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_2_5(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
568.93 0.6

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
569.45 2.5

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd ^{2,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_2_4--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
570.01 0.8

Annotations:

YZ redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd ^{2,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_2_4--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
597.27 32.9
598.42 3.2
601.39 0.6
612.47 1.0
615.4 20.4
616.33 3.0
631.49 1.3
639.51 1.4
655.48 1.0
655.97 1.1
656.29 2.2

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
657.34 39.3

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#zcleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
658.32 11.7
675.34 59.3

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p/#ycleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
676.26 9.3
676.54 6.6
692.34 1.5
697.31 0.9
699.33 0.9
713.97 0.6
714.37 1.1

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
715.43 2.2

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
716.36 15.0
717.35 5.4
732.36 34.3

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
733.34 25.9

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
734.32 7.8
735.43 1.3
752.46 4.2
753.15 0.9
753.53 0.9
765.32 0.7
775.61 1.1

Annotations:

^{0,2}A_{GalNAc}Y ?1D-GalNAc,p/#acleavage_0_2(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
782.36 1.4
783.47 1.7
785.31 4.0
786.49 2.2
793.98 1.2
794.4 4.8
800.16 2.6
800.46 1.9
803.4 25.7
804.34 8.6
825.09 0.7
826.34 1.4
842.6 3.2
843.57 1.3
848.47 1.0
849.46 1.1
856.4 0.5
860.36 1.9

Annotations:

Z redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#zcleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
861.41 3.8
862.53 0.7
878.73 2.6

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
879.3 5.7

Annotations:

Y redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p/#ycleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
879.62 3.1
880.53 1.6
886.36 0.7
896.28 1.3
904.5 0.6

Annotations:

^{2,5}A_{GalNAc} ?1D-GalNAc,p/#acleavage_2_5(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
912.45 1.0
916.77 1.1
917.43 2.4
943.8 0.8

There are 30 similar spectra:

Id Structure Score

3977

0.60

6813

0.60

5177

0.59

5183

0.59

7696

0.59

7684

0.59

7691

0.59

6814

0.59

7238

0.59

6592

0.58

7151

0.58

5521

0.58

3763

0.58

5443

0.58

5586

0.58

4208

0.58

7629

0.58

5796

0.58

7184

0.58

4083

0.58

7394

0.58

7423

0.58

5735

0.58

7175

0.58

3505

0.58

7628

0.58

4812

0.57

4245

0.57

5237

0.57

5207

0.57

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 1023.39
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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