Structure

    redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd

Rainbow trout mucus O-glycosylation

GlyTouCan Accession Number: Loading...

Peak Intensity
221.14 0.3

Annotations:

YY redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
233.04 0.2
235.54 0.3
261.5 0.1
262.37 0.3
262.96 0.4
263.26 0.1
264.39 0.1
275.94 0.2
281.2 0.6
317.02 0.4
323.26 0.1
341.2 0.5
346.86 0.1
347.24 0.4
348.01 0.1
362.44 0.3
364.35 0.9

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
365.01 0.2

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
365.5 0.2

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
366.22 0.5

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p/#zcleavage$MONO,Und,-H,0,redEnd
367.18 0.3
370.98 0.2
372.17 0.2
381.77 0.2

Annotations:

C ?1D-GalNAc,p/#ccleavage--??1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
384.42 0.2

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p/#ycleavage$MONO,Und,-H,0,redEnd
389.23 8.7

Annotations:

ZZ redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??1D-GlcNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
389.52 4.0

Annotations:

ZZ redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??1D-GlcNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd
390.2 1.7
396.31 0.1
400.47 0.6
406.99 1.0

Annotations:

YZ redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??1D-GlcNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
407.32 1.0

Annotations:

YZ redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??1D-GlcNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
410.24 0.3
413.87 0.3
424.54 0.8

Annotations:

YY redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd
438.29 0.3
456.23 0.1
474.56 0.6
509.34 3.7
510.43 0.6
521.32 1.6
522.56 0.1
527.21 5.6
527.44 4.7
528.36 1.6
533.11 0.2
533.38 0.1
533.88 0.2
541.41 0.4
544.42 0.2
545.15 0.3
545.34 0.2
550.18 0.4
550.91 0.3
551.23 0.2
551.53 0.2
562.08 0.3
568.16 0.4
569.33 115.3

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p--??1D-Gal,p/#zcleavage$MONO,Und,-H,0,redEnd Z redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
570.32 22.4
571.05 0.0
576.96 0.3
582.17 0.3
582.43 0.1
586.69 0.3

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
587.44 6.6

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
587.78 1.1

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
588.09 1.0

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p--??1D-Gal,p/#ycleavage$MONO,Und,-H,0,redEnd Y redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
588.48 1.9
589.06 0.0
602.45 0.2
605.6 0.3
607.08 0.2
627.69 0.5
647.49 0.4
651.39 0.4
657.18 0.5
660.09 1.3
661.3 0.5
669.83 0.3
671.37 0.1
672.54 0.9
675.01 0.1
676.41 0.6
676.64 0.1
689.27 6.5
689.6 1.8
689.93 0.0
690.47 3.5
690.74 0.6
704.17 0.6
705.43 0.3
705.71 0.2
707.44 1.7
707.69 1.2
708.45 1.0
712.52 0.3
713.57 0.1
716.91 0.2

There are 39 similar spectra:

Id Structure Score

0.99

5560

0.98

4206

0.98

3877

0.98

3118

0.98

3754

0.98

5428

0.97

0.97

4805

0.97

4237

0.97

5229

0.97

7732

0.97

3496

0.97

5200

0.97

0.97

0.97

7650

0.97

7666

0.97

5778

0.97

7624

0.97

4081

0.96

0.96

5711

0.96

7239

0.96

7991

0.95

3973

0.95

5178

0.93

5561

0.91

7404

0.90

7433

0.90

0.85

3119

0.85

0.83

4849

0.83

7625

0.78

3878

0.70

6719

0.67

7992

0.66

5712

0.52

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 748.29
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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