Recombinant mucin-type proteins carrying LacdiNAc on different O-glycan core chains fail to support H. pylori binding

Yolanda H. Mthembu , Chunsheng Jin , Médea Padra, Jining Liu , Niclas G. Karlsson, Sara K. Lindén , and Jan Holgersson, Manuscript

Retention Time Recorded Precursor Mass Structure
5.22 384.17 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-Gal,p$MONO,Und,-H,0,redEnd
5.22 384.17 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-GlcNAc,p--4b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
5.22 993.38 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-GlcNAc,p--4b1D-GalNAc,p(--3b1D-GlcNAc,p)--4b1D-Gal,p$MONO,Und,-H,0,redEnd
6.43 425.17 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
13.55 675.24 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
13.56 628.25 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-GlcNAc,p--4b1D-GalNAc,p$MONO,Und,-H,0,redEnd
14.11 587.23 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
16.02 790.3 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-Gal,p--3b1D-GlcNAc,p--4b1D-GalNAc,p$MONO,Und,-H,0,redEnd
16.25 691.24 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-Gal,p--3a2D-NeuGc,p$MONO,Und,-H,0,redEnd
16.33 675.24 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
16.74 749.28 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
16.99 749.28 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-Gal,p--3b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
19.01 790.3 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-GlcNAc,p--4b1D-Gal,p--3b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
19.51 982.33 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuGc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
19.68 966.34 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
20.31 1081.4 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6b1D-GlcNAc,p--4b1D-GalNAc,p$MONO,Und,-H,0,redEnd
21.62 894.32 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-GlcNAc,p--4b1D-Gal,p--3a2D-NeuGc,p$MONO,Und,-H,0,redEnd
21.81 1040.37 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
21.85 952.36 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-GlcNAc,p--4b1D-Gal,p--3b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
23.77 1114.41 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-Gal,p--3b1D-GlcNAc,p--4b1D-Gal,p--3b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
24.15 1040.37 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--4b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
25.21 1040.37 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-Gal,p--3b1D-GlcNAc,p--4b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
25.84 1284.48 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-GlcNAc,p--4b1D-GalNAc,p(--3b1D-GlcNAc,p)--4b1D-Gal,p}--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
26.5 673.23 [M -2H]2- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--4b1D-Gal,p}(--3a2D-NeuGc,p)--3a2D-NeuAc,p$MONO,Und,-2H,0,redEnd
26.83 1331.47 [M -1H]1- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6b1D-GlcNAc,p--4b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
30.11 1243.45 [M -1H]1- redEnd--?a1D-GalNAc,p--3b1D-GlcNAc,p--4b1D-Gal,p--3b1D-GlcNAc,p--4b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
31.4 847.79 [M -2H]2- redEnd--?a1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6b1D-GlcNAc,p--4b1D-Gal,p--3b1D-GlcNAc,p--4b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-2H,0,redEnd

Structure Format

SNFG Oxford Text

Biological Context

N/A

HPLC

Column Hypercarb

Method

  • Over 40 min gradient with 0.010 mL/min run rate
  • 10 mM NH4HCO3
  • 0-40% acetonitrile in 10 mM NH4HCO3

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE

See report

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