Structure

    redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd

Gill mucus and gill mucin O-glycosylation in healthy and amebic gill disease affected Atlantic salmon

GlyTouCan Accession Number: Loading...

Peak Intensity
202.09 22.5

Annotations:

B ?1D-GalNAc,p/#bcleavage--3a1D-GalNAc,p$MONO,Und,-H,0,redEnd
204.18 71.1

Annotations:

YZ redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p/#ycleavage)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p/#zcleavage)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
210.1 13.1
212.17 9.7
219.93 70.1

Annotations:

C ?1D-GalNAc,p/#ccleavage--3a1D-GalNAc,p$MONO,Und,-H,0,redEnd
222.03 31.4

Annotations:

YY redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p/#ycleavage)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
222.34 5.2
224.22 4.7
225.93 5.8
228.13 8.6
230.1 32.5
235.98 4.6
238.0 10.9
244.07 6.0
245.99 12.2
248.08 12.9
254.02 6.4
256.12 13.0
256.34 6.1
272.27 54.5
276.13 29.6
288.96 6.5
290.1 10664.9

Annotations:

B ?1D-GalNAc,p/#bcleavage--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
291.26 4.5
292.1 7.6

Annotations:

Y^{0,2}X_{NeuAc} redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p/#ycleavage)--6a2D-NeuAc,p/#xcleavage_0_2$MONO,Und,-H,0,redEnd
295.24 4.3
297.26 4.5
304.05 16.9
305.5 4.6
306.26 8.5
308.11 454.8

Annotations:

C ?1D-GalNAc,p/#ccleavage--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
310.1 6.0
316.08 5.1
333.92 11.3
336.15 4.6
339.26 9.1
343.25 10.0
346.14 6.9
346.93 8.3
347.27 11.0
348.13 5.3
350.17 4.8

Annotations:

^{0,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_4--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
357.26 5.7
365.32 83.6

Annotations:

^{0,4}X_{GalNAc} redEnd--??1D-GalNAc,p/#xcleavage_0_4--3a1D-GalNAc,p$MONO,Und,-H,0,redEnd
369.06 5.7
387.37 8.6
389.06 11.3
391.21 22.5
405.16 170.1
407.18 636.7

Annotations:

Z redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
409.24 11.3
409.71 5.4
417.3 20.0
425.21 1592.9

Annotations:

Y redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
429.18 130.8
431.29 14.0
433.27 43.6
435.23 39.1
441.17 5.8
447.28 30.0
449.32 17.5
451.23 160.3
459.31 28.3
465.18 6.1
467.23 132.7
469.26 15.3
477.2 573.7
493.12 11.1
494.44 13.9
495.19 409.5

Annotations:

Z redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p/#zcleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd ^{0,2}X_{NeuAc} redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p)--6a2D-NeuAc,p/#xcleavage_0_2$MONO,Und,-H,0,redEnd
496.0 6.8
496.99 8.1
504.46 5.4
513.21 68.5

Annotations:

Y redEnd--??1D-GalNAc,p(--3a1D-GalNAc,p/#ycleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
514.08 6.6
514.44 5.1
515.25 67.4
517.97 14.7
518.27 18.2
518.49 9.9
530.08 7.7
548.23 15.0
566.41 7.3
578.33 29.4
579.32 4.5
594.25 7.8
596.33 7.9
608.39 42.0
612.45 13.6
620.3 10.5
638.26 782.6
654.23 19.7
654.54 6.2
654.97 6.6
656.25 2122.7
670.29 4.5
672.05 8.9
672.41 5.5
674.28 20.1
680.25 214.5

There are 18 similar spectra:

Id Structure Score

4803

1.00

6800

1.00

5705

1.00

5773

1.00

6713

1.00

7422

0.99

7393

0.99

7182

0.99

7144

0.99

5772

0.98

5553

0.98

5704

0.97

3750

0.97

4845

0.96

7166

0.96

6712

0.96

5512

0.95

5513

0.90

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 715.28
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

Taxonomy - Salmo Salar
Tissue - Gills

HPLC

Column

Method

  • 40 min gradient with 10 ul/min run rate
  • 10 mM (NH4)HCO3 in water
  • 10 mM (NH4)HCO3 in 80% acetontirile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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