Structure

    redEnd--??1D-GalNAc,p(--3?1D-GalNAc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd

Rainbow trout mucus O-glycosylation

GlyTouCan Accession Number: Loading...

Peak Intensity
202.01 2.0

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1D-GalNAc,p$MONO,Und,-H,0,redEnd
203.93 6.3

Annotations:

ZY redEnd--??1D-GalNAc,p(--??1D-GalNAc,p/#zcleavage)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--??1D-GalNAc,p/#ycleavage)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
204.16 3.7

Annotations:

ZY redEnd--??1D-GalNAc,p(--??1D-GalNAc,p/#zcleavage)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd YZ redEnd--??1D-GalNAc,p(--??1D-GalNAc,p/#ycleavage)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
205.14 0.8
212.07 2.5
219.88 4.7

Annotations:

C ?1D-GalNAc,p/#ccleavage--??1D-GalNAc,p$MONO,Und,-H,0,redEnd
226.16 0.9
229.95 0.9
230.19 0.9
242.33 1.8
244.09 0.8
270.03 1.7
272.21 4.4
273.03 0.9
276.14 3.5
284.02 1.0
288.0 2.6
288.23 0.9
289.14 1.7

Annotations:

B ?1D-GalNAc,p/#bcleavage--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
290.1 828.3

Annotations:

B ?1D-GalNAc,p/#bcleavage--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
291.1 88.4
291.98 1.3
308.13 42.8

Annotations:

C ?1D-GalNAc,p/#ccleavage--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
309.11 6.4
310.47 1.1
321.13 0.8
330.21 0.9
339.1 1.2
347.22 3.7
357.31 0.9
365.2 7.6
389.29 1.9
405.22 10.5
406.36 1.3

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-GalNAc,p)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
407.25 49.0

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-GalNAc,p)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
408.37 3.5
417.26 3.0
418.12 1.9
425.25 112.6

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-GalNAc,p)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
426.21 17.2
429.28 8.6
430.19 2.4
431.04 0.9
431.35 2.7
433.14 1.2
435.18 1.3
435.46 0.9
451.27 9.7
451.49 5.3
452.54 0.8
459.36 1.5
466.07 1.0
467.23 8.3
468.39 1.0
469.39 1.6
477.21 44.4
477.69 3.9
478.27 8.3
492.86 1.1
493.25 3.3
494.3 10.1

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-GalNAc,p/#zcleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
495.29 35.4

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-GalNAc,p/#zcleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
496.3 8.0
500.06 1.2
513.29 20.2

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-GalNAc,p/#ycleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
514.3 26.0
515.46 3.6
516.36 1.8
518.36 1.4
534.29 0.9
548.47 1.2
549.45 0.9
560.28 1.2
570.28 0.9
578.26 2.2
579.11 0.8
579.41 1.3
590.3 1.8
591.19 0.9
595.52 1.3
596.42 1.2
597.38 1.1
608.31 2.5
608.57 0.9
612.59 2.2
620.12 2.7
620.39 1.8
638.31 79.6
639.35 25.5
646.97 0.8
647.2 0.8
654.52 1.8
655.43 6.4
656.3 169.6
657.3 41.0
672.55 1.1
674.15 0.8
680.55 13.2
681.58 1.8
878.46 0.8

There are 18 similar spectra:

Id Structure Score

7182

1.00

7422

1.00

7393

1.00

4803

0.99

5424

0.99

5705

0.99

6713

0.99

5773

0.99

6800

0.99

5553

0.98

5772

0.98

3750

0.97

5704

0.97

4845

0.96

6712

0.95

7166

0.95

5512

0.95

5513

0.89

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 715.28
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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