Structure

    redEnd--??1D-GalNAc,p(--3?1D-GalNAc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd

Rainbow trout mucus O-glycosylation

GlyTouCan Accession Number: Loading...

Peak Intensity
290.13 37.7

Annotations:

B ?1D-GalNAc,p/#bcleavage--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
290.9 0.0

Annotations:

B ?1D-GalNAc,p/#bcleavage--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
291.32 2.2
291.92 0.0
292.49 0.0
293.01 0.0
293.56 0.0
294.09 0.0
332.19 1.6

Annotations:

^{1,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_1_4--3?1D-GalNAc,p$MONO,Und,-H,0,redEnd
332.77 0.0

Annotations:

^{1,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_1_4--3?1D-GalNAc,p$MONO,Und,-H,0,redEnd
333.28 0.0

Annotations:

^{1,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_1_4--3?1D-GalNAc,p$MONO,Und,-H,0,redEnd
334.1 2.5

Annotations:

^{1,4}A_{GalNAc} ?1D-GalNAc,p/#acleavage_1_4--3?1D-GalNAc,p$MONO,Und,-H,0,redEnd
334.66 0.0
335.2 0.0
335.73 0.0
336.28 0.0
336.8 0.0
372.21 1.0
372.77 0.0
373.29 0.0
373.86 0.0
374.37 0.0
407.35 1.5

Annotations:

Z redEnd--??1D-GalNAc,p(--3?1D-GalNAc,p)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
407.92 0.0

Annotations:

Z redEnd--??1D-GalNAc,p(--3?1D-GalNAc,p)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
408.43 0.0
409.01 0.0
409.51 0.0
425.29 5.5

Annotations:

Y redEnd--??1D-GalNAc,p(--3?1D-GalNAc,p)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
425.71 0.0

Annotations:

Y redEnd--??1D-GalNAc,p(--3?1D-GalNAc,p)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
426.06 0.5

Annotations:

Y redEnd--??1D-GalNAc,p(--3?1D-GalNAc,p)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
426.62 0.0
427.19 0.0
427.7 0.0
428.25 0.0
451.49 1.0
452.04 0.0
452.6 0.0
453.12 0.0
453.69 0.0
466.19 3.0
466.72 0.0
467.27 0.0
467.79 0.0
468.36 0.0
468.87 0.0
477.27 1.0
477.83 0.0
478.36 0.0
478.89 0.0
479.44 0.0
513.06 1.5

Annotations:

Y redEnd--??1D-GalNAc,p(--3?1D-GalNAc,p/#ycleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
513.61 0.0

Annotations:

Y redEnd--??1D-GalNAc,p(--3?1D-GalNAc,p/#ycleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
514.15 0.0

Annotations:

Y redEnd--??1D-GalNAc,p(--3?1D-GalNAc,p/#ycleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
514.69 0.0
515.22 0.0
515.77 0.0
526.78 1.1
527.36 0.0
527.87 0.0
528.44 0.0
528.95 0.0
548.39 6.1
548.94 0.0
549.47 0.0
550.02 0.0
550.55 0.0
551.11 0.0
575.27 0.9
575.8 0.0
576.36 0.0
576.88 0.0
577.44 0.0
593.18 3.5
593.72 0.0
594.27 0.0
594.79 0.0
595.36 0.0
595.87 0.0
638.13 2.6
638.69 0.0
639.2 0.0
639.77 0.0
640.28 0.0
640.84 0.0
646.98 0.6
647.53 0.0
648.07 0.0
648.61 0.0
649.14 0.0
656.22 2.0
656.78 0.0
657.3 0.0
657.86 0.0
658.38 0.0
658.94 0.0
673.46 0.5
674.02 0.0
674.54 0.0
675.1 0.0
675.62 0.0

There are 18 similar spectra:

Id Structure Score

5705

0.96

5424

0.96

4803

0.96

6800

0.96

6713

0.96

7422

0.96

7393

0.96

5773

0.96

7182

0.95

7144

0.95

5772

0.95

4845

0.95

5553

0.95

3750

0.95

5704

0.94

6712

0.93

5512

0.91

5513

0.89

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 715.28
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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