Structure

    redEnd--??1D-GalNAc,p(--3?1D-GalNAc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd

Rainbow trout mucus O-glycosylation

GlyTouCan Accession Number: Loading...

Peak Intensity
204.19 4.2

Annotations:

YZ redEnd--??1D-GalNAc,p(--3?1D-GalNAc,p/#ycleavage)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd ZY redEnd--??1D-GalNAc,p(--3?1D-GalNAc,p/#zcleavage)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
218.15 1.0
219.92 3.3

Annotations:

C ?1D-GalNAc,p/#ccleavage--3?1D-GalNAc,p$MONO,Und,-H,0,redEnd
220.18 1.4

Annotations:

C ?1D-GalNAc,p/#ccleavage--3?1D-GalNAc,p$MONO,Und,-H,0,redEnd
223.26 1.5
224.11 0.8
229.8 1.0
230.21 0.7
231.87 0.7
271.98 5.2
272.25 2.4
272.89 1.1
276.16 3.5
287.9 1.3
289.18 2.2

Annotations:

B ?1D-GalNAc,p/#bcleavage--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
290.13 582.4

Annotations:

B ?1D-GalNAc,p/#bcleavage--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
291.13 61.5
292.27 0.7
308.13 24.9

Annotations:

C ?1D-GalNAc,p/#ccleavage--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
309.2 4.5
317.29 0.7
330.24 1.5
339.43 1.0
340.3 1.1
346.15 1.1
365.29 3.3
383.26 0.9
391.47 0.9
405.17 3.8
405.4 1.8
405.81 1.3
407.23 36.9

Annotations:

Z redEnd--??1D-GalNAc,p(--3?1D-GalNAc,p)--6a2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
408.18 3.2
408.42 2.1
410.0 0.8
417.25 4.1
418.28 1.0
425.26 93.4

Annotations:

Y redEnd--??1D-GalNAc,p(--3?1D-GalNAc,p)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
426.1 7.1

Annotations:

Y redEnd--??1D-GalNAc,p(--3?1D-GalNAc,p)--6a2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
426.42 7.6
429.15 2.9
429.42 1.8
430.42 1.7
431.31 1.3
431.78 0.8
433.29 2.4
434.84 1.5
435.36 1.8
446.95 1.8
447.41 2.0
451.44 3.7
452.69 1.0
459.08 0.7
467.29 4.7
469.48 2.1
477.33 24.5
478.31 6.4
493.52 1.7
494.12 1.7
494.46 0.9

Annotations:

Z redEnd--??1D-GalNAc,p(--3?1D-GalNAc,p/#zcleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
495.43 11.3

Annotations:

Z redEnd--??1D-GalNAc,p(--3?1D-GalNAc,p/#zcleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
496.28 5.1
496.67 1.9
496.93 0.9
500.48 0.7
513.31 13.3

Annotations:

Y redEnd--??1D-GalNAc,p(--3?1D-GalNAc,p/#ycleavage)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
514.48 14.1
515.52 2.9
518.13 1.3
520.39 0.8
520.97 1.1
526.25 1.0
537.32 0.8
548.52 3.3
552.34 1.2
555.34 0.8
578.44 2.2
594.72 1.5
608.28 2.2
608.52 1.4
613.57 0.7

Annotations:

^{0,2}A_{GalNAc} ?1D-GalNAc,p/#acleavage_0_2(--3?1D-GalNAc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
630.49 1.2
638.4 52.2
639.2 8.3
639.49 9.0
647.34 1.0
653.16 0.8
654.26 2.1
654.65 1.1
655.43 9.5
656.36 121.6
657.34 26.0
658.52 0.8
672.43 1.1
675.18 1.1
680.44 18.1
681.34 2.4
681.62 2.6
842.27 1.1
1225.49 0.9

There are 22 similar spectra:

Id Structure Score

7144

1.00

7393

1.00

7422

1.00

7988

0.99

6713

0.99

5424

0.99

6800

0.99

5773

0.99

5705

0.98

4803

0.98

0.98

5772

0.97

5553

0.97

4845

0.96

0.96

7987

0.96

7166

0.95

5704

0.95

6712

0.95

3750

0.95

5512

0.94

5513

0.92

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 715.28
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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