Structure

    redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd

Rainbow trout mucus O-glycosylation

GlyTouCan Accession Number: Loading...

Peak Intensity
251.08 0.4
256.02 0.6
271.82 0.5
288.14 0.5
289.27 0.7

Annotations:

B ?1D-GalNAc,p/#bcleavage--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
290.12 24.5

Annotations:

B ?1D-GalNAc,p/#bcleavage--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
290.65 3.6

Annotations:

B ?1D-GalNAc,p/#bcleavage--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
291.16 5.5
306.15 1.2
308.16 1.7

Annotations:

C ?1D-GalNAc,p/#ccleavage--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
309.25 1.0
311.11 0.4
339.06 0.9
364.11 0.3

Annotations:

B ?1D-GalNAc,p/#bcleavage--??1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
383.46 1.2

Annotations:

YY redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
384.25 2.4

Annotations:

YY redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
435.04 0.3
438.26 0.4
463.31 0.7
469.47 0.4
477.04 1.6
477.32 2.4
477.59 0.6
478.41 0.8
479.17 0.7
479.49 2.1
493.22 0.4
494.35 1.4

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
495.12 0.4

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
495.47 1.1

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
510.71 0.4
512.72 1.1

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
513.36 2.9

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
513.65 2.3

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
514.25 1.4
527.56 1.0
528.22 1.6
539.44 0.7
545.14 0.6
556.49 0.6
560.31 0.5
569.29 8.8

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p)--??2D-NeuAc,p/#zcleavage$MONO,Und,-H,0,redEnd
587.3 34.0

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p)--??2D-NeuAc,p/#ycleavage$MONO,Und,-H,0,redEnd
588.26 7.2
589.63 0.5
595.64 0.4
597.15 1.2
598.21 1.1
598.43 0.8
613.47 0.8
615.1 1.0
616.04 1.9
629.32 0.9
639.39 1.9
639.67 0.5
640.38 0.3
647.31 1.4
657.58 8.4

Annotations:

Z redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p/#zcleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
658.24 2.2
658.6 1.1
671.16 1.0
674.24 0.5
675.4 11.0

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
676.13 2.8

Annotations:

Y redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p/#ycleavage)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
676.46 11.1
677.17 1.2
677.46 3.1
680.24 0.5
689.09 1.2
697.32 0.6
703.37 0.4
740.13 1.9
741.32 0.9
749.18 0.8
758.2 1.1
759.35 0.6
760.39 0.7
769.03 0.8
776.3 0.3
778.59 1.0
781.97 1.1
785.21 0.5
792.31 0.5
800.47 7.8
801.49 2.2
816.58 0.6
817.38 1.2
817.68 1.9
818.38 73.2
819.45 13.1
824.32 1.4
832.9 0.9
836.13 0.6
836.46 0.4
840.23 1.2
841.23 0.5
841.6 1.1
842.36 5.7
843.49 0.9
851.02 0.7

There are 17 similar spectra:

Id Structure Score

6806

0.95

5435

0.95

4858

0.92

5722

0.81

5436

0.78

7409

0.78

7438

0.78

5721

0.77

5785

0.74

4808

0.74

7149

0.74

4859

0.70

7408

0.61

7437

0.61

5520

0.61

5570

0.59

6805

0.50

Structure Format

SNFG Oxford Text

Structure details

Reducing Mass: 877.33
(Calculated Monoisotopic)

Reducing End: Reduced

Persubstitution: Underivatised

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE Structural data

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