The O-glycome of human nigrostriatal tissue and its alteration in Parkinsons disease

Hayden Wilkinson, Kristina A.Thomsson, Ana L. Rebelo, Mark Hilliard, Abhay Pandit, Pauline M. Rudd, Niclas G. Karlsson, Radka Saldova, Manuscript

Retention Time Recorded Precursor Mass Structure
13.61 384.27 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p$MONO,Und,-H,0,redEnd
15.96 464.25 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p--3?1S$MONO,Und,-H,0,redEnd
16.87 384.27 [M -1H]1- redEnd--??1D-GalNAc,p--3a1D-Gal,p$MONO,Und,-H,0,redEnd
17.01 472.4 [M -1H]1- redEnd--??1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
17.09 692.42 [M -1H]1- redEnd--??1D-Man,p--2b1D-GlcNAc,p(--4b1D-Gal,p)--3a1L-Fuc,p$MONO,Und,-H,0,redEnd
17.3 513.42 [M -1H]1- redEnd--??1D-GalNAc,p--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
17.53 425.4 [M -1H]1- redEnd--??1D-GalNAc,p--??1D-GlcNAc,p$MONO,Und,-H,0,redEnd
17.53 464.25 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p--6?1S$MONO,Und,-H,0,redEnd
17.7 675.5 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
17.79 601.42 [M -1H]1- redEnd--??2D-NeuAc,p--?a2D-NeuAc,p$MONO,Und,-H,0,redEnd
18.23 772.33 [M -1H]1- redEnd--??1D-Man,p--??1D-GlcNAc,p((--??1D-Gal,p)--??1L-Fuc,p)--??1S$MONO,Und,-H,0,redEnd
18.23 895.5 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p(--4b1D-Gal,p)--3a1L-Fuc,p$MONO,Und,-H,0,redEnd
18.87 755.33 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
19.3 675.25 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p--3a2D-NeuAc,p$MONO,Und,-H,0,redEnd
19.31 546.42 [M -1H]1- redEnd--??1D-Man,p--2b1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
19.66 1046.33 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p(--??2D-NeuAc,p)--??1S)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
19.84 966.5 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
20.02 755.33 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??2D-NeuAc,p--??1S$MONO,Und,-H,0,redEnd
20.49 1046.42 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p(--??2D-NeuAc,p)--??1S)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
22.4 1040.58 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6b1D-GlcNAc,p--4b1D-Gal,p$MONO,Und,-H,0,redEnd
24.46 1040.58 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p--4b1D-Gal,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
24.63 837.63 [M -1H]1- redEnd--??1D-Man,p--??1D-GlcNAc,p--??1D-Gal,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
24.73 802.33 [M -1H]1- redEnd--??1D-Man,p--??1D-GlcNAc,p--??1D-Gal,p(--??1HexA,p)--??1S$MONO,Und,-H,0,redEnd
26.23 1331.42 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6b1D-GlcNAc,p--4b1D-Gal,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd

Structure Format

SNFG Oxford Text

Biological Context

Taxonomy - Homo Sapiens
Tissue - Brain

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE

See report

UniCarb-DR Creative Commons License

Supported by SIB ExPASy  |  JST / NBDCDICP