Sea water temperature affects mucin O-glycosylation in Atlantic salmon (Salmo salar)

Kristina A. Thomsson, John Benktander, Binyam S. Dagnachew, Lene Sveen, Macarena P. Quintana-Hayashi, Ulrike Böcker, Lill-Heidi Johansen, Mette Breiland, Celeste Jacq, Elisabeth Ytteborg, Sara Linden, Manuscript

Retention Time Recorded Precursor Mass Structure
12.74 472.41 [M -1H]1- redEnd--??1D-GalNAc,p--??2D-KDN,p$MONO,Und,-H,0,redEnd
13.99 425.19 [M -1H]1- redEnd--??1D-GalNAc,p--??1D-GlcNAc,p$MONO,Und,-H,0,redEnd
14.23 464.08 [M -1H]1- redEnd--??1D-GalNAc,p--??1D-Gal,p--??1S$MONO,Und,-H,0,redEnd
14.65 529.28 [M -1H]1- redEnd--??1D-GalNAc,p--??2D-NeuGc,p$MONO,Und,-H,0,redEnd
15.13 513.25 [M -1H]1- redEnd--??1D-GalNAc,p--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
15.28 384.2 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p$MONO,Und,-H,0,redEnd
16.46 425.21 [M -1H]1- redEnd--??1D-GalNAc,p--??1D-GalNAc,p$MONO,Und,-H,0,redEnd
16.55 587.21 [M -1H]1- redEnd--??1D-GalNAc,p--??1D-GalNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
16.83 675.35 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
17.55 691.35 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??2D-NeuGc,p$MONO,Und,-H,0,redEnd
17.9 1098.43 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1D-GalNAc,p--??1L-Fuc,p)--??1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
17.9 464.08 [M -1H]1- redEnd--??1D-GalNAc,p--??1D-Gal,p--??1S$MONO,Und,-H,0,redEnd
18.55 571.29 [M -1H]1- redEnd--??1D-GalNAc,p--??1D-GalNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
19.07 755.26 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
19.24 790.31 [M -1H]1- redEnd--??1D-GalNAc,p--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p$MONO,Und,-H,0,redEnd
19.51 675.35 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
19.66 862.35 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-GalNAc,p--??1L-Fuc,p)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
19.81 878.4 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-GalNAc,p--??1D-Gal,p)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
19.81 749.41 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
20.04 966.25 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p--??2D-NeuAc,p)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
20.11 732.31 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-GalNAc,p)--??2D-NeuGc,p$MONO,Und,-H,0,redEnd
20.2 716.13 [M -1H]1- redEnd--??1D-GalNAc,p(--3?1D-GalNAc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
20.58 894.36 [M -1H]1- redEnd--??1D-GalNAc,p--??1D-GalNAc,p(--??1D-Gal,p)--??2D-NeuGc,p$MONO,Und,-H,0,redEnd
20.68 878.4 [M -1H]1- redEnd--??1D-GalNAc,p--??1D-GalNAc,p(--??1D-Gal,p)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
21.02 952.46 [M -1H]1- redEnd--??1D-GalNAc,p--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
21.49 894.33 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-GalNAc,p--??1D-Gal,p)--??2D-NeuGc,p$MONO,Und,-H,0,redEnd
23.8 1040.36 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p--??2D-NeuAc,p)--??1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
27.64 705.53 [M -2H]2- redEnd--??1D-GalNAc,p(--??1D-Gal,p--??2D-NeuAc,p)--??1D-GlcNAc,p(--??1D-Gal,p--??2D-NeuAc,p)--??1S$MONO,Und,-2H,0,redEnd
28.91 1331.27 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p--??2D-NeuAc,p)--??1D-GlcNAc,p--??1D-Gal,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd

Structure Format

SNFG Oxford Text

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

N/A

MIRAGE

See report

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