Rainbow trout mucus O-glycosylation

Kristina A. Thomsson, John Benktander , Sinan Sharba , Henrik Sundh , Kristina Sundell , and Sara K. Lindén , Manuscript

Retention Time Recorded Precursor Mass Structure
9.27 384.2 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p$MONO,Und,-H,0,redEnd
10.54 384.25 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p$MONO,Und,-H,0,redEnd
10.82 513.2 [M -1H]1- redEnd--??1D-GalNAc,p--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
11.97 587.2 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
12.23 675.2 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
12.53 529.19 [M -1H]1- redEnd--??1D-GalNAc,p--??2D-NeuGc,p$MONO,Und,-H,0,redEnd
12.63 513.42 [M -1H]1- redEnd--??1D-GalNAc,p--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
13.0 425.2 [M -1H]1- redEnd--??1D-GalNAc,p--??1D-GalNAc,p$MONO,Und,-H,0,redEnd
13.17 587.2 [M -1H]1- redEnd--??1D-GalNAc,p--??1D-GalNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
13.34 587.2 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6b1D-GlcNAc,p$MONO,Und,-H,0,redEnd
13.64 587.2 [M -1H]1- redEnd--??1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p$MONO,Und,-H,0,redEnd
13.71 691.2 [M -1H]1- redEnd--??1D-GalNAc,p--??1D-Gal,p--??2D-NeuGc,p$MONO,Und,-H,0,redEnd
13.82 675.2 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
14.38 755.2 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
14.43 675.2 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
14.87 571.2 [M -1H]1- redEnd--??1D-GalNAc,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
15.23 716.3 [M -1H]1- redEnd--??1D-GalNAc,p(--3?1D-GalNAc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
15.41 691.2 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??2D-NeuGc,p$MONO,Und,-H,0,redEnd
15.41 804.3 [M -1H]1- redEnd--??1D-GalNAc,p--??2D-NeuAc,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
15.64 675.2 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
15.67 384.25 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p$MONO,Und,-H,0,redEnd
15.75 878.3 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
15.98 732.3 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-GalNAc,p)--6a2D-NeuGc,p$MONO,Und,-H,0,redEnd
16.04 733.3 [M -1H]1- redEnd--??1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
16.1 966.3 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p--??2D-NeuAc,p)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
16.17 716.3 [M -1H]1- redEnd--??1D-GalNAc,p(--3?1D-GalNAc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
16.29 878.3 [M -1H]1- redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
16.56 966.3 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
16.56 966.3 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p--??2D-NeuAc,p)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
16.61 936.4 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--6b1HexNAc,p$MONO,Und,-H,0,redEnd
17.31 1024.4 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
17.49 1081.4 [M -1H]1- redEnd--??1D-GalNAc,p(--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
17.64 1227.5 [M -1H]1- redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
17.76 1139.4 [M -1H]1- redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??1HexNAc,p$MONO,Und,-H,0,redEnd
17.98 1535.6 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p--3a2D-NeuAc,p)--6b1D-GlcNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
18.2 1007.4 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-GalNAc,p)--6a2D-NeuAc,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
18.4 587.2 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6?1HexNAc,p$MONO,Und,-H,0,redEnd
18.59 464.1 [M -1H]1- redEnd--??1D-GalNAc,p--??1D-Gal,p--??1S$MONO,Und,-H,0,redEnd
18.69 1169.4 [M -1H]1- redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??2D-NeuAc,p)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
18.79 513.2 [M -1H]1- redEnd--??1D-GalNAc,p--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
18.86 1227.5 [M -1H]1- redEnd--??1D-GalNAc,p(--??1HexNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
18.86 1227.5 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??2D-NeuAc,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
19.34 895.3 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p(--??1D-Gal,p)--??1L-Fuc,p$MONO,Und,-H,0,redEnd
19.73 675.2 [M -1H]1- redEnd--??1D-GalNAc,p(--3b1D-Gal,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
21.17 790.3 [M -1H]1- redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p)--??1HexNAc,p$MONO,Und,-H,0,redEnd
21.38 749.3 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p)--??1D-GlcNAc,p--??1D-Gal,p$MONO,Und,-H,0,redEnd
21.56 733.2 [M -1H]1- redEnd--??1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
22.24 733.2 [M -1H]1- redEnd--??1D-GalNAc,p--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p$MONO,Und,-H,0,redEnd
22.3 675.2 [M -1H]1- redEnd--??1D-GalNAc,p--3b1D-Gal,p--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
22.34 936.4 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??1HexNAc,p$MONO,Und,-H,0,redEnd
22.79 936.4 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??1HexNAc,p$MONO,Und,-H,0,redEnd
23.07 755.2 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p--??1S)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd
23.07 716.3 [M -1H]1- redEnd--??1D-GalNAc,p(--3?1D-GalNAc,p)--6a2D-NeuAc,p$MONO,Und,-H,0,redEnd
23.12 1139.4 [M -1H]1- redEnd--??1D-GalNAc,p--??1HexNAc,p(--??1D-Gal,p--??1HexNAc,p--??1L-Fuc,p)--??1HexNAc,p$MONO,Und,-H,0,redEnd
24.34 966.3 [M -1H]1- redEnd--??1D-GalNAc,p(--??1D-Gal,p--??2D-NeuAc,p)--??2D-NeuAc,p$MONO,Und,-H,0,redEnd

Structure Format

SNFG Oxford Text

Biological Context

N/A

HPLC

Column

Method

  • 78 min gradient with approx 3-5 ul/min (static split from 250 ul/min) run rate
  • 10 mM (NH4)HCO3
  • 10 mM (NH4)HCO3 in 80% acetonitrile

Mass Spec

Thermo Fisher LTQ Linear Ion Trap ESI

MIRAGE

See report

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